Issue 40, 2017

The development of cell-initiated degradable hydrogel based on methacrylated alginate applicable to multiple microfabrication technologies

Abstract

Biomimetic multicellular complex tissues can be obtained via 3D microfabrication approaches, although the lack of active materials that meet the needs of both molding and the maintenance of cell function is the main bottleneck in their application. The development of 3D cell culture platforms, which are suitable for multiple cell types and various microfabrication technologies, would open the way to the biomimetic construction of multicellular complex tissues. In this study, a MMP-sensitive photocrosslinkable hydrogel system based on methacrylated alginate, which is suitable for the survival of multiple cell types and can be employed in a variety of micro-manufacturing technologies, was designed for the construction of 3D microfeatures to meet both the demands of molding and the requirement for biomimetic function. By employing the characteristics of photocrosslinking and ion crosslinking, the gel system was combined with a variety of microfabrication techniques to construct biomimetic microstructures with a spatial distribution of multiple types of cell. The diversity of shapes and cell types in these assemblies, as well as the maintenance of cell activity, collectively show that the functional MMP-sensitive gel system has the potential to be used as a cell culture platform for the construction of tissue microstructures.

Graphical abstract: The development of cell-initiated degradable hydrogel based on methacrylated alginate applicable to multiple microfabrication technologies

Supplementary files

Article information

Article type
Paper
Submitted
30 मई 2017
Accepted
15 सितम्बर 2017
First published
16 सितम्बर 2017

J. Mater. Chem. B, 2017,5, 8060-8069

The development of cell-initiated degradable hydrogel based on methacrylated alginate applicable to multiple microfabrication technologies

J. Sun, D. Wei, K. Yang, Y. Yang, X. Liu, H. Fan and X. Zhang, J. Mater. Chem. B, 2017, 5, 8060 DOI: 10.1039/C7TB01458A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements