Photodynamic hemostatic silk fibroin film with photo-controllable modulation on macrophage for bacteria infected wound healing

Abstract

Massive hemorrhage and chronic wounds caused by bacterial infections after trauma have always been significant challenges in clinical practice. An ideal hemostatic wound dressing should not only simultaneously manage bleeding and prevent bacterial infections, but also holds excellent biocompatibility and bioactivities to successfully modulate immune microenvironments to promote wound healing. Previously, a silk fibroin based light responsive film was successfully prepared. In this study, the silk fibroin film was demonstrated to possess effective capacity of light-induced non-compressible hemostasis on liver hemorrhage and tail bleeding in vivo by binding with blood platelets to promote the clotting cascade. Significantly, the films exhibited photo-controllable modulation activity on macrophage through repeated near-infrared irradiation to regulate the immune microenvironment to enhance photodynamic antibacterial therapy. Moreover, the light responsive silk fibroin film effectively promoted Staphylococcus aureus infected burn wound healing in vivo, providing a powerful strategy for wound healing of burns.