Issue 12, 2024

A multi-channel electrochemical biosensor based on polyadenine tetrahedra for the detection of multiple drug resistance genes

Abstract

Antimicrobial resistance poses a serious threat to human health due to the high morbidity and mortality caused by drug-resistant microbial infections. Therefore, the development of rapid, sensitive and selective identification methods is key to improving the survival rate of patients. In this paper, a sandwich-type electrochemical DNA biosensor based on a polyadenine-DNA tetrahedron probe was constructed. The key experimental conditions were optimized, including the length of polyadenine, the concentration of the polyadenine DNA tetrahedron, the concentration of the signal probe and the hybridization time. At the same time, poly-avidin-HRP80 was used to enhance the electrochemical detection signal. Finally, excellent biosensor performance was achieved, and the detection limit for the synthetic DNA target was as low as 1 fM. In addition, we verified the practicability of the system by analyzing E. coli with the MCR-1 plasmid and realized multi-channel detection of the drug resistance genes MCR-1, blaNDM, blaKPC and blaOXA. With the ideal electrochemical interface, the polyA-based biosensor exhibits excellent stability, which provides powerful technical support for the rapid detection of antibiotic-resistant strains in the field.

Graphical abstract: A multi-channel electrochemical biosensor based on polyadenine tetrahedra for the detection of multiple drug resistance genes

Supplementary files

Article information

Article type
Paper
Submitted
29 מרץ 2024
Accepted
22 אפר 2024
First published
28 אפר 2024

Analyst, 2024,149, 3425-3432

A multi-channel electrochemical biosensor based on polyadenine tetrahedra for the detection of multiple drug resistance genes

Y. Song, J. Feng, X. Wang, Y. Wen, L. Xu, Y. Huo, L. Wang, Q. Tao, Z. Yang, G. Liu, M. Chen, L. Li and J. Yan, Analyst, 2024, 149, 3425 DOI: 10.1039/D4AN00488D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements