Issue 9, 2023

Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy

Abstract

Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function. Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification. Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further. Sugars, however, are difficult analytes to probe due to their isomeric nature. In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes. The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides. Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.

Graphical abstract: Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
26 אוק 2022
Accepted
05 מרץ 2023
First published
21 מרץ 2023

Analyst, 2023,148, 2035-2044

Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy

H. C. Schorr and Z. D. Schultz, Analyst, 2023, 148, 2035 DOI: 10.1039/D2AN01762H

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