Issue 16, 2022

Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

Abstract

This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

Graphical abstract: Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

Article information

Article type
Paper
Submitted
10 מרץ 2022
Accepted
30 מאי 2022
First published
31 מאי 2022

Lab Chip, 2022,22, 2971-2977

Author version available

Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

K. Takahashi, S. Chida, T. Suwatthanarak, M. Iida, M. Zhang, M. Fukuyama, M. Maeki, A. Ishida, H. Tani, T. Yasui, Y. Baba, A. Hibara, M. Okochi and M. Tokeshi, Lab Chip, 2022, 22, 2971 DOI: 10.1039/D2LC00224H

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