Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives

Congcong Chen; Shuaishuai Wang; Madhusudhan Reddy Gadi; He Zhu; Fei Liu; Chang-Cheng Liu; Lei Li; Fengshan Wang; Peixue Ling; Hongzhi Cao

doi:10.1039/D0CC03268A

Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives†

Congcong Chen,^abc Shuaishuai Wang,

^d Madhusudhan Reddy Gadi,

^d He Zhu,^d Fei Liu,^ac Chang-Cheng Liu,*^be Lei Li,

*^d Fengshan Wang,*^ab Peixue Ling*^ac and Hongzhi Cao

*^be

Author affiliations

* Corresponding authors

^a School of Pharmaceutical Sciences, Key Laboratory of Chemical Biology (Ministry of Education), Cheeloo College of Medicine, Shandong University, Jinan 250012, China
E-mail: fswang@sdu.edu.cn

^b National Glycoengineering Research Center, State Key Laboratory of Microbial Technology, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, Qingdao 266237, China
E-mail: ccliu@sdu.edu.cn, hzcao@sdu.edu.cn

^c Shandong Academy of Pharmaceutical Science, Key Laboratory of Biopharmaceuticals, Engineering Laboratory of Poly-Saccharide Drugs, Jinan 250101, China
E-mail: lingpeixue@126.com

^d Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA
E-mail: lli22@gsu.edu

^e Laboratory for Marine Drugs and Bioproducts, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China

Abstract

A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly-N-acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.

This article is part of the themed collection: Chemical Communications HOT Articles

Chemical Communications

Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives†

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Enzymatic modular synthesis and microarray assay of poly-N-acetyllactosamine derivatives

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