Issue 40, 2017

Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles

Abstract

Biological fluids contain a plethora of different components that can coat nanoparticle's surface and critically affect their interaction with living systems. The recent literature has focused efforts on understanding the overall protein role in this nanoparticle's coating (commonly called a protein corona) without considering other components’ influence. Here, we report a protocol to study the relationship between biological fluid components excluding proteins (referred to as a biomolecular corona) and nanoparticles. For this purpose, functionalized silica nanoparticles were duly synthesized to experimentally investigate the extent of biomolecular corona adsorption over different surfaces. This adsorption was directly correlated with the silica surface chemical constitution, while the extent of biomolecular corona coating affected the overall surface charge and the colloidal stability of the functionalized nanoparticles. In addition, the bactericidal properties of biomolecular corona-coated nanoparticles were tested against two medically relevant bacteria (Escherichia coli and Staphylococcus aureus). Biological results indicated that the presence and the extent of the biomolecular corona affect the bactericidal properties of the nanoparticles.

Graphical abstract: Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
27 יונ 2017
Accepted
22 אוג 2017
First published
22 אוג 2017

J. Mater. Chem. B, 2017,5, 8052-8059

Biomolecular corona formation: nature and bactericidal impact on surface-modified silica nanoparticles

M. Emer and M. B. Cardoso, J. Mater. Chem. B, 2017, 5, 8052 DOI: 10.1039/C7TB01744H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements