Issue 20, 2023

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Abstract

Genetically encoded biosensors based on fluorescent proteins (FPs) are powerful tools for tracking analytes and cellular events with high spatial and temporal resolution in living cells and organisms. Compared with intensiometric readout and ratiometric readout, fluorescence lifetime readout provides absolute measurements, independent of the biosensor expression level and instruments. Thus, genetically encoded fluorescence lifetime biosensors play a vital role in facilitating accurate quantitative assessments within intricate biological systems. In this review, we first provide a concise description of the categorization and working mechanism of genetically encoded fluorescence lifetime biosensors. Subsequently, we elaborate on the combination of the fluorescence lifetime imaging technique and lifetime analysis methods with fluorescence lifetime biosensors, followed by their application in monitoring the dynamics of environment parameters, analytes and cellular events. Finally, we discuss worthwhile considerations for the design, optimization and development of fluorescence lifetime-based biosensors from three representative cases.

Graphical abstract: Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Article information

Article type
Critical Review
Submitted
14 יול 2023
Accepted
26 אוג 2023
First published
29 אוג 2023

Analyst, 2023,148, 4939-4953

Genetically encoded fluorescence lifetime biosensors: overview, advances, and opportunities

Y. Mo, H. Zhou, J. Xu, X. Chen, L. Li and S. Zhang, Analyst, 2023, 148, 4939 DOI: 10.1039/D3AN01201H

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