Issue 17, 2023

Fluorescence detection of 4-nitrophenol and α-glucosidase activity based on 4-nitrophenol-regulated fluorescence of silicon nanoparticles

Abstract

A fluorescence assay for the detection of 4-nitrophenol (4-NP), α-glucosidase (α-Glu) activity and α-Glu inhibitors (AGIs) is developed based on the inner filter effect (IFE), a flexible and simple signal transfer strategy. In this assay, silicon nanoparticles (Si NPs) synthesized under mild and easily accessible conditions are employed as fluorescent indicators. 4-NP efficaciously quenches the fluorescence of Si NPs through the IFE at a very rapid rate, thus achieving 4-NP detection in a mix-to-read manner, which is suitable for on-site detection. The quenching mechanism has been comprehensively studied and confirmed. More significantly, based on the fact that 4-NP can be generated through α-Glu-catalyzed hydrolysis of 4-nitrophenyl-α-D-glucopyranoside (NPG), the fluorescence detection of α-Glu activity is legitimately achieved by employing NPG as the substrate. The linear ranges for 4-NP and α-Glu activity detection are 0.5–60 μM and 0.5–60 mU mL−1 with low detection limits of 0.074 μM and 0.094 mU mL−1, respectively. This method not only can preciously assay targets in real samples, but is also capable of screening AGIs as drugs as well as assessing their inhibition efficiency.

Graphical abstract: Fluorescence detection of 4-nitrophenol and α-glucosidase activity based on 4-nitrophenol-regulated fluorescence of silicon nanoparticles

Supplementary files

Article information

Article type
Paper
Submitted
14 יונ 2023
Accepted
11 יול 2023
First published
12 יול 2023

Analyst, 2023,148, 4030-4036

Fluorescence detection of 4-nitrophenol and α-glucosidase activity based on 4-nitrophenol-regulated fluorescence of silicon nanoparticles

F. Liu, F. Liang, Z. Li, G. Kang, T. Wang, C. Chen and Y. Lu, Analyst, 2023, 148, 4030 DOI: 10.1039/D3AN00966A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements