Issue 14, 2023

Advances in deoxyribonucleic acid extraction techniques and point-of-care molecular diagnosis of foodborne pathogens

Abstract

A conventional molecular assay-based point-of-care (POC) diagnostic test involves three major stages: deoxyribonucleic acid (DNA) extraction, amplification, and amplicon detection. Among these steps, DNA extraction is costly and time-consuming. Nevertheless, it is a crucial step for the identification of sensitive and specific diseases. This review summarizes the advantages and disadvantages of DNA extraction methods over the past 10 years to effectively implement POC pathogen testing in the future. The first section briefly explains the necessity of DNA extraction and molecular assays for food pathogen detection. The second section extensively discusses DNA extraction based on liquid–liquid extraction, solid-phase extraction, and electrophoretic techniques. Molecular assay-based methods and a few commercially available POC devices for the detection of foodborne pathogens are detailed in the third and fourth sections. Finally, present challenges and future perspectives for the fabrication of integrated POC devices are highlighted.

Graphical abstract: Advances in deoxyribonucleic acid extraction techniques and point-of-care molecular diagnosis of foodborne pathogens

Article information

Article type
Tutorial Review
Submitted
09 ינו 2023
Accepted
28 מאי 2023
First published
31 מאי 2023

Analyst, 2023,148, 3153-3168

Advances in deoxyribonucleic acid extraction techniques and point-of-care molecular diagnosis of foodborne pathogens

R. Sivakumar and N. Y. Lee, Analyst, 2023, 148, 3153 DOI: 10.1039/D3AN00045A

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