Issue 5, 2022

Rapid cell pairing and fusion based on oscillating bubbles within an acoustofluidic device

Abstract

Cell fusion is an essential event in many biological processes and has gained increasing attention in the field of biotechnology. In this study, we demonstrate an effective and convenient strategy for cell capture, pairing, and fusion based on oscillating bubbles within an acoustofluidic device. Multirectangular structures of the same size were fabricated at the sidewall of polydimethylsiloxane to generate monodisperse microbubbles. These microbubbles oscillated with a similar amplitude under single-frequency acoustic excitation. Cells were simultaneously captured and paired on the surface of the oscillating bubbles within 40 ms, and the efficiency reached approximately 90%. Homotypic or heterotypic cell membrane fusion was achieved within 15 and 20 min, respectively. More importantly, the homotypic fused cells enabled migration and proliferation at 24 h, indicating that the important biological functions were not altered.

Graphical abstract: Rapid cell pairing and fusion based on oscillating bubbles within an acoustofluidic device

Supplementary files

Article information

Article type
Paper
Submitted
28 נוב 2021
Accepted
03 פבר 2022
First published
09 פבר 2022

Lab Chip, 2022,22, 921-927

Rapid cell pairing and fusion based on oscillating bubbles within an acoustofluidic device

X. Liu, W. Zhang, U. Farooq, N. Rong, J. Shi, N. Pang, L. Xu, L. Niu and L. Meng, Lab Chip, 2022, 22, 921 DOI: 10.1039/D1LC01074C

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