Issue 5, 2019

Reversible fluorescence modulation of BSA stabilised copper nanoclusters for the selective detection of protamine and heparin

Abstract

Protamine and heparin are the most important polyionic drugs used during surgeries and extracorporeal therapies. In this article, a selective and sensitive fluorescence method for the detection of both protamine and heparin was developed by using bovine serum albumin stabilised copper nanoclusters. Blue emitting fluorescent copper nanoclusters were synthesized in aqueous solution using bovine serum albumin as a capping agent and a reducing agent. A one pot microwave assisted method was adopted to synthesize fluorescent copper nanoclusters showing emission at 410 nm upon excitation at 330 nm. The fluorescence of copper nanoclusters was found to be enhanced after the addition of protamine and the limit of detection obtained is 0.12 ng mL−1. The significant enhancement in fluorescence can be attributed to the electrostatic interactions between the copper nanocluster and protamine. In contrast, the enhanced fluorescence intensity of the copper nanocluster with protamine added was decreased after the addition of heparin, and the copper nanocluster regained its original fluorescence intensity. This can be attributed to the strong interaction of protamine with heparin and the limit of detection was calculated as 0.0406 ng mL−1. The selectivity and sensitivity of the sensor for both protamine and heparin were also determined in the presence of potentially co-existing biomolecules, cations, and anions and satisfactory results were obtained. Additionally the validity of the proposed protamine and heparin sensor was attested in real sample matrices such as human urine samples and human blood serum samples. The results exhibited that the recovery percentage of protamine and heparin reached 98–99% and 92–99% in urine samples and 97–99% in serum samples.

Graphical abstract: Reversible fluorescence modulation of BSA stabilised copper nanoclusters for the selective detection of protamine and heparin

Supplementary files

Article information

Article type
Paper
Submitted
03 ספט 2018
Accepted
14 דצמ 2018
First published
21 דצמ 2018

Analyst, 2019,144, 1799-1808

Reversible fluorescence modulation of BSA stabilised copper nanoclusters for the selective detection of protamine and heparin

R. S. Aparna, J. S. Anjali Devi, R. R. Anjana, J. Nebu and S. George, Analyst, 2019, 144, 1799 DOI: 10.1039/C8AN01703D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements