Red and far-red cleavable fluorescent dyes for self-labelling enzyme protein tagging and interrogation of GPCR co-internalization

Kilian Roßmann, Ramona Birke, Joshua Levitz, Ben Jones and Johannes Broichhagen

RSC Chem. Biol., 2025, 6, 11
DOI: 10.1039/D4CB00209A

Bioisostere-conjugated fluorescent probes for live-cell protein imaging without non-specific organelle accumulation

Takuya Kamikawa, Akari Hashimoto, Nozomi Yamazaki, Junya Adachi, Ayami Matsushima, Kazuya Kikuchi and Yuichiro Hori

Chem. Sci., 2024, 15, 8097
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N-Cyanorhodamines: cell-permeant, photostable and bathochromically shifted analogues of fluoresceins

Lukas Heynck, Jessica Matthias, Mariano L. Bossi, Alexey N. Butkevich and Stefan W. Hell

Chem. Sci., 2022, 13, 8297
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Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy

Michael Trumpp, Anna Oliveras, Hannes Gonschior, Julia Ast, David J. Hodson, Petra Knaus, Martin Lehmann, Melissa Birol and Johannes Broichhagen

Chem. Commun., 2022, 58, 13724
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Revealing the tissue-level complexity of endogenous glucagon-like peptide-1 receptor expression and signaling

Julia Ast, Daniela Nasteska, Nicholas H. F. Fine, Daniel J. Nieves, Zsombor Koszegi, Yann Lanoiselée, Federica Cuozzo, Katrina Viloria, Andrea Bacon, Nguyet T. Luu, Philip N. Newsome, Davide Calebiro, Dylan M. Owen, Johannes Broichhagen and David J. Hodson

Nat Commun, 2023, 14
DOI: 10.1038/s41467-022-35716-1

Melanocortin System Activates Carotid Body Arterial Chemoreceptors in Hypertension

Audrys G. Pauza, Pratik Thakkar, Xin Shen, Igor S.A. Felippe, Kilian Roßmann, Manami Oya, Fiona D. McBryde, Kazuhiro Nakamura, Johannes Broichhagen, David J. Hodson, Dainius H. Pauza, David Murphy and Julian F.R. Paton

Circulation Research, 2025, 137, 967
DOI: 10.1161/CIRCRESAHA.125.326394

MRAP2 potentiates GPCR signaling by conserved mechanisms that are disrupted by obesity-associated genetic variants

Aqfan Jamaluddin, Rachael A. Wyatt, Johannes Broichhagen, Joshua Levitz and Caroline M. Gorvin

Cell Reports, 2026, 45, 117018
DOI: 10.1016/j.celrep.2026.117018

Distinct beta-arrestin coupling and intracellular trafficking of metabotropic glutamate receptor homo- and heterodimers

Joon Lee, Alberto J. Gonzalez-Hernandez, Melanie Kristt, Nohely Abreu, Kilian Roßmann, Anisul Arefin, Dagan C. Marx, Johannes Broichhagen and Joshua Levitz

Sci. Adv., 2023, 9
DOI: 10.1126/sciadv.adi8076

Setting up multicolour TIRF microscopy down to the single molecule level

Chiara Schirripa Spagnolo and Stefano Luin

Biomolecular Concepts, 2023, 14
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Expanded LUXendin Color Palette for GLP1R Detection and Visualization In Vitro and In Vivo

Julia Ast, Alissa N. Novak, Tom Podewin, Nicholas H. F. Fine, Ben Jones, Alejandra Tomas, Ramona Birke, Kilian Roßmann, Bettina Mathes, Jenny Eichhorst, Martin Lehmann, Amelia K. Linnemann, David J. Hodson and Johannes Broichhagen

JACS Au, 2022, 2, 1007
DOI: 10.1021/jacsau.2c00130

Fluorescent GLP1R/GIPR dual agonist probes reveal cell targets in the pancreas and brain

Anne de Bray, Anna G. Roberts, Sarah Armour, Jason Tong, Christiane Huhn, Blaise Gatin-Fraudet, Kilian Roßmann, Ali H. Shilleh, Wanqing Jiang, Natalie S. Figueredo Burgos, James P. P. Trott, Katrina Viloria, Daniela Nasteska, Abigail Pearce, Satsuki Miyazaki, Jeremy W. Tomlinson, Dylan M. Owen, Daniel J. Nieves, Julia Ast, Malgorzata Cyranka, Alexey Epanchintsev, Carina Ämmälä, Frank Reimann, Tolga Soykan, Graham Ladds, Alice E. Adriaenssens, Stefan Trapp, Ben Jones, Johannes Broichhagen and David J. Hodson

Nat Metab, 2025, 7, 1536
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Entwicklung von chemischen Initiatoren der Dimerisierung von HaloTag und SNAP‐Tag zur Untersuchung von Signaltransduktion induziert durch Rezeptor‐Oligomerisierung

Michael Trumpp, Blaise Gatin‐Fraudet, Kjell Bruckmann, Wiktor Burdzinski, Kilian Roßmann, Joshua Levitz, Petra Knaus, Jerome Jatzlau and Johannes Broichhagen

Angewandte Chemie, 2025, 137
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HaloTag‐Based Reporters for Fluorescence Imaging and Biosensing

Alexander Cook, Franziska Walterspiel and Claire Deo

ChemBioChem, 2023, 24
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Multicolor, Cell-Impermeable, and High Affinity BACE1 Inhibitor Probes Enable Superior Endogenous Staining and Imaging of Single Molecules

Florian Stockinger, Pascal Poc, Alexander Möhwald, Sandra Karch, Stephanie Häfner, Christian Alzheimer, Guillaume Sandoz, Tobias Huth and Johannes Broichhagen

J. Med. Chem., 2024, 67, 10152
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Localized GLP1 receptor pre-internalization directs pancreatic alpha cell to beta cell communication

Jason C.L. Tong, Charlotte Frazer-Morris, Ali H. Shilleh, Katrina Viloria, Anne de Bray, Adithya Muraleedaran Nair, Paul R.V. Johnson, Rebecca Spiers, Ahmad Kobiita, Oladapo E. Olaniru, Shanta J. Persaud, Robert Hauffe, André Kleinridders, Carsten Schultz, C. Bruce Verchere, Canqi Cui, Jonathan E. Campbell, Malgorzata Cyranka, Alexey Epanchintsev, Carina Ämmälä, Johannes Broichhagen and David J. Hodson

Cell Metabolism, 2025, 37, 1698
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Harnessing Cyanine‐like Properties to Develop Bright Fluorogenic Probes Based on Viscosity‐Sensitive Molecular Rotors

Blaise Dumat and Carolina Chieffo

Chemistry A European J, 2025, 31
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Developing HaloTag and SNAP‐Tag Chemical Inducers of Dimerization to Probe Receptor Oligomerization and Downstream Signaling

Michael Trumpp, Blaise Gatin‐Fraudet, Kjell Bruckmann, Wiktor Burdzinski, Kilian Roßmann, Joshua Levitz, Petra Knaus, Jerome Jatzlau and Johannes Broichhagen

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202506830

Fluorogenic Chemical Probes for Wash-free Imaging of Cell Membrane Damage in Ferroptosis, Necrosis, and Axon Injury

Philipp Mauker, Daniela Beckmann, Annabel Kitowski, Constanze Heise, Chantal Wientjens, Andrew J. Davidson, Simone Wanderoy, Gabin Fabre, Angelika B. Harbauer, Will Wood, Christoph Wilhelm, Julia Thorn-Seshold, Thomas Misgeld, Martin Kerschensteiner and Oliver Thorn-Seshold

J. Am. Chem. Soc., 2024
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Controlling Intramolecular Rotation with Five-Membered Heterocycles Facilitates the Design of Highly Cell-Permeable Xanthene-Based Fluorogenic Probes

Shahi Imam Reja, Yuichiro Hori, Youhei Takeda, Miyako Nishiura, Masafumi Minoshima and Kazuya Kikuchi

J. Am. Chem. Soc., 2025, 147, 47997
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Design of Bright Chemogenetic Reporters Based on the Combined Engineering of Fluorogenic Molecular Rotors and of the HaloTag Protein

Justine Coïs, Sylvestre P. J. T. Bachollet, Louis Sanchez, Nicolas Pietrancosta, Vincent Vialou, Jean‐Maurice Mallet and Blaise Dumat

Chemistry A European J, 2024, 30
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The accessory protein MRAP2 directly interacts with melanocortin-3 receptor to enhance signaling

Aqfan Jamaluddin, Rachael A. Wyatt, Joon Lee, Georgina K. C. Dowsett, John A. Tadross, Johannes Broichhagen, Giles S. H. Yeo, Joshua Levitz and Caroline M. Gorvin

Sci. Signal., 2025, 18
DOI: 10.1126/scisignal.adu4315

SNAPpa: A Photoactivatable SNAP-tag for the Spatiotemporal Control of Protein Labeling

Sabrina Mandl, Barbara Maiwald, Elena Adlmanninger, Ramona Birke, Sandra Schlee, Adam Pruška, Philipp Bittner, Renato Zenobi, Tolga Soykan, Gerti Beliu, Johannes Broichhagen and Andrea Hupfeld

JACS Au, 2025, 5, 3589
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A general one-step protocol to generate impermeable fluorescent HaloTag substrates for in situ live cell application and super-resolution imaging

Kilian Roßmann, Ulrich Pabst, Bianca C. Baciu, Siqi Sun, Christiane Huhn, Christina Holmboe Olesen, Maria Kowald, Eleni Tapp, Marie Bieck, Ramona Birke, Brenda C. Shields, Pyeonghwa Jeong, Jiyong Hong, Michael R. Tadross, Joshua Levitz, Martin Lehmann, Noa Lipstein and Johannes Broichhagen

Nat Commun, 2026, 17
DOI: 10.1038/s41467-025-68134-0

openFrame: A modular, sustainable, open microscopy platform with single‐shot, dual‐axis optical autofocus module providing high precision and long range of operation

J. Lightley, S. Kumar, M. Q. Lim, E. Garcia, F. Görlitz, Y. Alexandrov, T. Parrado, C. Hollick, E. Steele, K. Roßmann, J. Graham, J. Broichhagen, I. A. McNeish, C. A. Roufosse, M. A. A. Neil, C. Dunsby and P. M. W. French

Journal of Microscopy, 2023, 292, 64
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Fluorescent labeling of live-cell surfaceome and its application in antibody-target interaction analysis

Haoting Wang, Ying Zhang, Wenchao Wang, Jinning Shao, Rizwan Ullah Khan, Su Zeng and Linghui Qian

Analytica Chimica Acta, 2024, 1330, 343296
DOI: 10.1016/j.aca.2024.343296

Toolbox of Clickable Benzylguanines for Labeling of HoxB8-Derived Macrophages via SNAP-Tag and Bioorthogonal Chemistry

Jonas Schöning, Lukas Rösner, Dominic Alexej Depke, Sabine Hüwel, Anastasiia Kukhar, Michael Schäfers and Andrea Rentmeister

Bioconjugate Chem., 2025, 36, 34
DOI: 10.1021/acs.bioconjchem.4c00364

Endogenous SNAP-Tagging of Munc13-1 for Monitoring Synapse Nanoarchitecture

Maria Kowald, Sylvestre P. J. T. Bachollet, Fritz Benseler, Maria Steinecker, Moritz Boll, Sofia Kaushik, Tolga Soykan, Siqi Sun, Ramona Birke, Dragana Ilic, Nils Brose, Hanna Hörnberg, Martin Lehmann, Silvio O. Rizzoli, Johannes Broichhagen and Noa Lipstein

JACS Au, 2025, 5, 2475
DOI: 10.1021/jacsau.4c00946