Laser capture microdissection and native mass spectrometry for spatially-resolved analysis of intact protein assemblies in tissue

James W. Hughes, Emma K. Sisley, Oliver J. Hale and Helen J. Cooper

Chem. Sci., 2024, 15, 5723
DOI: 10.1039/D3SC04933G

Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications

Tian Xu, Qianjie Wang, Qianyi Wang and Liangliang Sun

Anal. Methods, 2024, 16, 4664
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Enhanced Spatial Mapping of Histone Proteoforms in Human Kidney Through MALDI-MSI by High-Field UHMR-Orbitrap Detection

Kevin J. Zemaitis, Dušan Veličković, William Kew, Kyle L. Fort, Maria Reinhardt-Szyba, Annapurna Pamreddy, Yanli Ding, Dharam Kaushik, Kumar Sharma, Alexander A. Makarov, Mowei Zhou and Ljiljana Paša-Tolić

Anal. Chem., 2022, 94, 12604
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Top-Down Proteomics Analysis of Picogram-Level Complex Samples Using Spray-Capillary-Based Capillary Electrophoresis–Mass Spectrometry

Zhitao Zhao, Yanting Guo, Trishika Chowdhury, Samin Anjum, Jiaxue Li, Lushuang Huang, Kellye A. Cupp-Sutton, Anthony Burgett, Dingjing Shi and Si Wu

Anal. Chem., 2024, 96, 8763
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Mapping and quantifying neuropeptides in the enteric nervous system

Alex González-Vergara, Benjamín Benavides and Marcela Julio-Pieper

Journal of Neuroscience Methods, 2023, 393, 109882
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Capillary Electrophoresis–Mass Spectrometry for Top-Down Proteomics

Qianjie Wang, Qianyi Wang, Guijie Zhu and Liangliang Sun

Annual Review of Analytical Chemistry, 2025, 18, 125
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High‐Throughput Proteoform Imaging for Revealing Spatial‐Resolved Changes in Brain Tissues Associated with Alzheimer's Disease

Yue Sun, Dan Liu, Yu Liang, Xue Yang, Xinxin Liu, Baofeng Zhao, Zhen Liang, Yukui Zhang and Lihua Zhang

Advanced Science, 2025, 12
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Spatially Resolved Top-Down Proteomics of Tissue Sections Based on a Microfluidic Nanodroplet Sample Preparation Platform

Yen-Chen Liao, James M. Fulcher, David J. Degnan, Sarah M. Williams, Lisa M. Bramer, Dušan Veličković, Kevin J. Zemaitis, Marija Veličković, Ryan L. Sontag, Ronald J. Moore, Ljiljana Paša-Tolić, Ying Zhu and Mowei Zhou

Molecular & Cellular Proteomics, 2023, 22, 100491
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Top‐Down Proteomics Study of Aging and Sexual Dimorphism of Zebrafish Brains

Mehrdad Falamarzi Askarani, William Poulos, Maryam Rahimzadeh Dashtaki, Seyed Amirhossein Sadeghi, Jose B. Cibelli, Fei Fang and Liangliang Sun

J Mass Spectrom, 2026, 61
DOI: 10.1002/jms.70009

Buffering agents and additives for the background electrolyte solutions used for peptide and protein capillary zone electrophoresis

Danilo Corradini

TrAC Trends in Analytical Chemistry, 2023, 164, 117080
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Spatial top-down proteomics for the functional characterization of human kidney

Kevin J. Zemaitis, James M. Fulcher, Rashmi Kumar, David J. Degnan, Logan A. Lewis, Yen-Chen Liao, Marija Veličković, Sarah M. Williams, Ronald J. Moore, Lisa M. Bramer, Dušan Veličković, Ying Zhu, Mowei Zhou and Ljiljana Paša-Tolić

Clin Proteom, 2025, 22
DOI: 10.1186/s12014-025-09531-x

Influence of different sample preparation approaches on proteoform identification by top-down proteomics

Philipp T. Kaulich, Kyowon Jeong, Oliver Kohlbacher and Andreas Tholey

Nat Methods, 2024, 21, 2397
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Properties, Origin, and Consistency of Truncated Proteoforms Across Top-Down Proteomic Studies

Philipp T. Kaulich, James M. Fulcher and Andreas Tholey

Molecular & Cellular Proteomics, 2025, 24, 101465
DOI: 10.1016/j.mcpro.2025.101465

Biological mass spectrometry enables spatiotemporal ‘omics: From tissues to cells to organelles

Leena R. Pade, Kaitlyn E. Stepler, Erika P. Portero, Kellen DeLaney and Peter Nemes

Mass Spectrometry Reviews, 2024, 43, 106
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Identification of proteoforms by top‐down proteomics using two‐dimensional low/low pH reversed‐phase liquid chromatography‐mass spectrometry

Philipp T. Kaulich, Liam Cassidy and Andreas Tholey