Online dashboards for SARS-CoV-2 wastewater data need standard best practices: An environmental health communication agenda

Colleen C. Naughton, Rochelle H. Holm, Nancy J. Lin, Brooklyn P. James and Ted Smith

Journal of Water and Health, 2023, 21, 615
DOI: 10.2166/wh.2023.312

Recommendations for Advancing FAIR and Open Data Standards in the Water Treatment Community

Amanda N. Quay, Peter S. Fiske and Meagan S. Mauter

ACS EST Eng., 2022, 2, 337
DOI: 10.1021/acsestengg.1c00245

Comparing Rates of Change in SARS-CoV-2 Wastewater Load and Clinical Cases in 19 Sewersheds Across Four Major Metropolitan Areas in the United States

Mitham Al-Faliti, Nadine Kotlarz, Camille McCall, Angela R. Harris, Adam L. Smith, Lauren B. Stadler, Francis L. de los Reyes and Jeseth Delgado Vela

ACS EST Water, 2022, 2, 2233
DOI: 10.1021/acsestwater.2c00106

Human norovirus (HuNoV) GII RNA in wastewater solids at 145 United States wastewater treatment plants: comparison to positivity rates of clinical specimens and modeled estimates of HuNoV GII shedders

Alexandria B. Boehm, Marlene K. Wolfe, Bradley J. White, Bridgette Hughes, Dorothea Duong, Niaz Banaei and Amanda Bidwell

J Expo Sci Environ Epidemiol, 2024, 34, 440
DOI: 10.1038/s41370-023-00592-4

Wastewater Detection of Emerging Arbovirus Infections: Case Study of Dengue in the United States

Marlene K. Wolfe, Abigail Harvey Paulos, Alessandro Zulli, Dorothea Duong, Bridgette Shelden, Bradley J. White and Alexandria B. Boehm

Environ. Sci. Technol. Lett., 2024, 11, 9
DOI: 10.1021/acs.estlett.3c00769

Detection of Hemagglutinin H5 Influenza A Virus RNA and Model of Potential Inputs in an Urban California Sewershed

Abigail P. Paulos, Stephen P. Hilton, Bridgette Shelden, Dorothea Duong, Alexandria B. Boehm and Marlene K. Wolfe

Environ. Sci. Technol., 2025, 59, 16168
DOI: 10.1021/acs.est.4c14792

A scoping review of the private sector involvement in wastewater and environmental surveillance

Kavya Boyina, Rochelle H. Holm, Himeshi Samarasinghe, Petros Kingstone Chigwechokha, Ted Smith, John R. Cosgrove and Adam J. Gushgari

Discov Water, 2025, 5
DOI: 10.1007/s43832-025-00293-z

Influence of storage conditions and multiple freeze-thaw cycles on N1 SARS-CoV-2, PMMoV, and BCoV signal

Isha Thapar, Laura M. Langan, Haley Davis, R. Sean Norman, Heidi K. Bojes and Bryan W. Brooks

Science of The Total Environment, 2023, 896, 165098
DOI: 10.1016/j.scitotenv.2023.165098

Antiviral Phytoremediation for Sustainable Wastewater Treatment

Diaiti Zure, Aleksandra Drizo, Meng-Hau Sung, Amanuel Mehari, Eko Maiguo and David H-W Kuo

Sustainability, 2026, 18, 523
DOI: 10.3390/su18010523

Assessing the Impact of Wastewater Flow Rates on SARS‐CoV‐2 RNA Detection: Insights for Environmental Surveillance and Policymaking

André Felipe Bendix, Alex Batista Trentin, Deborah Catharine De Assis Leite, Betty Cristiane Kuhn, Felipe Beijamini, Simone Wendt, Flavia Regina Oliveira de Barros, Naiana Cristine Gabiatti, Juliana Morini Küpper Cardoso and Nédia de Castilhos Ghisi

Environmental Quality Mgmt, 2026, 35
DOI: 10.1002/tqem.70341

Diurnal Variability of SARS-CoV-2 RNA Concentrations in Hourly Grab Samples of Wastewater Influent during Low COVID-19 Incidence

Lorelay Mendoza Grijalva, Blake Brown, Amanda Cauble and William A. Tarpeh

ACS EST Water, 2022, 2, 2125
DOI: 10.1021/acsestwater.2c00061

Wastewater-Based Epidemiology of SARS-CoV-2 and Other Respiratory Viruses: Bibliometric Tracking of the Last Decade and Emerging Research Directions

Hassan Waseem, Rameesha Abid, Jafar Ali, Claire J. Oswald and Kimberley A. Gilbride

Water, 2023, 15, 3460
DOI: 10.3390/w15193460

Respiratory Syncytial Virus (RSV) RNA in Wastewater Settled Solids Reflects RSV Clinical Positivity Rates

Bridgette Hughes, Dorothea Duong, Bradley J. White, Krista R. Wigginton, Elana M. G. Chan, Marlene K. Wolfe and Alexandria B. Boehm

Environ. Sci. Technol. Lett., 2022, 9, 173
DOI: 10.1021/acs.estlett.1c00963

Building a framework for reproducibility: the case for standardized data reporting and metadata integration in zebrafish research

Mee S. Ngu, Sabrina Toro, Alexa Burger and Khai C. Ang

Disease Models & Mechanisms, 2025, 18
DOI: 10.1242/dmm.052441

Detection and quantification of human immunodeficiency virus-1 (HIV-1) total nucleic acids in wastewater settled solids from two California communities

Marlene K. Wolfe, Meri R. J. Varkila, Alessandro Zulli, Julie Parsonnet, Alexandria B. Boehm and Christopher A. Elkins

Appl Environ Microbiol, 2024, 90
DOI: 10.1128/aem.01477-24

Looking Forward: The Role of Academic Researchers in Building Sustainable Wastewater Surveillance Programs

Catherine Hoar, Jill McClary-Gutierrez, Marlene K. Wolfe, Aaron Bivins, Kyle Bibby, Andrea I. Silverman and Sandra L. McLellan

Environ Health Perspect, 2022, 130
DOI: 10.1289/EHP11519

Detection of SARS-CoV-2 Variants Mu, Beta, Gamma, Lambda, Delta, Alpha, and Omicron in Wastewater Settled Solids Using Mutation-Specific Assays Is Associated with Regional Detection of Variants in Clinical Samples

Marlene Wolfe, Bridgette Hughes, Dorothea Duong, Vikram Chan-Herur, Krista R. Wigginton, Bradley J. White, Alexandria B. Boehm and Nicole R. Buan

Appl Environ Microbiol, 2022, 88
DOI: 10.1128/aem.00045-22

Wastewater-Based Detection of Two Influenza Outbreaks

Marlene K. Wolfe, Dorothea Duong, Kevin M. Bakker, Michelle Ammerman, Lindsey Mortenson, Bridgette Hughes, Peter Arts, Adam S. Lauring, William J. Fitzsimmons, Emily Bendall, Calvin E. Hwang, Emily T. Martin, Bradley J. White, Alexandria B. Boehm and Krista R. Wigginton

Environ. Sci. Technol. Lett., 2022, 9, 687
DOI: 10.1021/acs.estlett.2c00350

Show us the data: global COVID-19 wastewater monitoring efforts, equity, and gaps

Colleen C Naughton, Fernando A Roman, Ana Grace F Alvarado, Arianna Q Tariqi, Matthew A Deeming, Krystin F Kadonsky, Kyle Bibby, Aaron Bivins, Gertjan Medema, Warish Ahmed, Panagis Katsivelis, Vajra Allan, Ryan Sinclair and Joan B Rose

FEMS Microbes, 2023, 4
DOI: 10.1093/femsmc/xtad003

Persistence of respiratory, enteric, and fecal indicator viruses in fecal sludge from on-site sanitation in Dakar, Senegal

Lorelay Mendoza Grijalva, Alsane Seck, Laura Roldan-Hernandez, Katherine E. Graham, Alexandria B. Boehm and William A. Tarpeh

Journal of Water, Sanitation and Hygiene for Development, 2024, 14, 916
DOI: 10.2166/washdev.2024.011

Spatially Informed Wastewater Differentiation among Locations during an Ongoing Measles Outbreak in Texas, USA

Laura M. Langan, Fallon L. Bain, Christine C. Snow, Janelle Oldfather, Olivia Sagvold, Kacie Kaneshiro, Charlotte Nwagwu, HyeongYoung Choi, Adam R. Wronski, Md Alamin, R. Sean Norman, Audrey Robertson, Layla Lustri, Victoria Salinas, Heidi K. Bojes and Bryan W. Brooks

ACS Environ. Au, 2025, 5, 543
DOI: 10.1021/acsenvironau.5c00122

Observations of Respiratory Syncytial Virus (RSV) Nucleic Acids in Wastewater Solids Across the United States in the 2022–2023 Season: Relationships with RSV Infection Positivity and Hospitalization Rates

Alessandro Zulli, Meri R.J. Varkila, Julie Parsonnet, Marlene K. Wolfe and Alexandria B. Boehm

ACS EST Water, 2024, 4, 1657
DOI: 10.1021/acsestwater.3c00725

Estimating the COVID-19 prevalence from wastewater

Jan Mohring, Neele Leithäuser, Jarosław Wlazło, Marvin Schulte, Maximilian Pilz, Johanna Münch and Karl-Heinz Küfer

Sci Rep, 2024, 14
DOI: 10.1038/s41598-024-64864-1

Monitoring SARS-CoV-2 RNA in Wastewater with RT-qPCR and Chip-Based RT-dPCR: Sewershed-Level Trends and Relationships to COVID-19

Daniel Ma, Judith Straathof, Yijing Liu and Natalie Marie Hull

ACS EST Water, 2022, 2, 2084
DOI: 10.1021/acsestwater.2c00055

Prospective study of Candida auris nucleic acids in wastewater solids in 190 wastewater treatment plants in the United States suggests widespread occurrence

Alessandro Zulli, Elana M. G. Chan, Bridgette Shelden, Dorothea Duong, Xiang-Ru S. Xu, Bradley J. White, Marlene K. Wolfe, Alexandria B. Boehm and Valerie J. Harwood

mBio, 2024, 15
DOI: 10.1128/mbio.00908-24

Moving beyond Wastewater: Perspectives on Environmental Surveillance of Infectious Diseases for Public Health Action in Low-Resource Settings

Jeseth Delgado Vela, Sarah E. Philo, Joe Brown, Mami Taniuchi, Molly Cantrell, Alexandra Kossik, Maya Ramaswamy, Sitara SR Ajjampur, Fatma Z. Guerfali, Rochelle H. Holm, John Scott Meschke, Maria Catherine B. Otero, Amy J. Pickering, Mahbubur Rahman, Alexander G. Shaw, Abhilasha Shrestha, Kwanrawee Sirikanchana, Vivi Maketa Tevuzula, Rolf U. Halden, Alexandria B. Boehm and Kyle Bibby

Environ. Health, 2024, 2, 684
DOI: 10.1021/envhealth.4c00119

What is eDNA method standardisation and why do we need it?

Susanna Theroux, Adam Sepulveda, Cathryn L. Abbott, Zachary Gold, Alison W. Watts, Margaret E. Hunter, Katy E. Klymus, Shana Lee Hirsch, Joseph M. Craine, Devin N. Jones, Rachel J. Brown, Joshua A. Steele, Miwa Takahashi, Rachel T. Noble and John A. Darling

MBMG, 2025, 9
DOI: 10.3897/mbmg.9.132076

The Tomato Brown Rugose Fruit Virus Movement Protein Gene Is a Novel Microbial Source Tracking Marker

Aravind Natarajan, Brayon J. Fremin, Danica T. Schmidtke, Marlene K. Wolfe, Soumaya Zlitni, Katherine E. Graham, Erin F. Brooks, Christopher J. Severyn, Kathleen M. Sakamoto, Norman J. Lacayo, Scott Kuersten, Jeff Koble, Glorianna Caves, Inna Kaplan, Upinder Singh, Prasanna Jagannathan, Andrew R. Rezvani, Ami S. Bhatt, Alexandria B. Boehm and Nicole R. Buan

Appl Environ Microbiol, 2023, 89
DOI: 10.1128/aem.00583-23

Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

Michael D. Parkins, Bonita E. Lee, Nicole Acosta, Maria Bautista, Casey R. J. Hubert, Steve E. Hrudey, Kevin Frankowski, Xiao-Li Pang and Graeme N. Forrest

Clin Microbiol Rev, 2024, 37
DOI: 10.1128/cmr.00103-22

Regional Replacement of SARS-CoV-2 Variant Omicron BA.1 with BA.2 as Observed through Wastewater Surveillance

Alexandria B. Boehm, Bridgette Hughes, Marlene K. Wolfe, Bradley J. White, Dorothea Duong and Vikram Chan-Herur

Environ. Sci. Technol. Lett., 2022, 9, 575
DOI: 10.1021/acs.estlett.2c00266

Augmentation of wastewater-based epidemiology with machine learning to support global health surveillance

Eva Aßmann, Timo Greiner, Hugues Richard, Matthew Wade, Shelesh Agrawal, Fabian Amman, Sindy Böttcher, Susanne Lackner, Markus Landthaler, Serghei Mangul, Viorel Munteanu, Fotis Psomopoulos, Maureen Smith, Maria Trofimova, Alexander Ullrich, Max von Kleist, Emanuel Wyler, Martin Hölzer and Christopher Irrgang

Nat Water, 2025, 3, 753
DOI: 10.1038/s44221-025-00444-5

Wastewater surveillance for bacterial targets: current challenges and future goals

Sarah E. Philo, Kara B. De León, Rachel T. Noble, Nicolette A. Zhou, Rashed Alghafri, Itay Bar-Or, Amanda Darling, Nishita D'Souza, Oumaima Hachimi, Devrim Kaya, Sooyeol Kim, Katrin Gaardbo Kuhn, Blythe A. Layton, Cresten Mansfeldt, Bethany Oceguera, Tyler S. Radniecki, Jeffrey L. Ram, Lauren P. Saunders, Abhilasha Shrestha, Lauren B. Stadler, Joshua A. Steele, Bradley S. Stevenson, Jason R. Vogel, Kyle Bibby, Alexandria B. Boehm, Rolf U. Halden, Jeseth Delgado Vela and Christopher A. Elkins

Appl Environ Microbiol, 2024, 90
DOI: 10.1128/aem.01428-23

Wastewater concentrations of human influenza, metapneumovirus, parainfluenza, respiratory syncytial virus, rhinovirus, and seasonal coronavirus nucleic-acids during the COVID-19 pandemic: a surveillance study

Alexandria B Boehm, Bridgette Hughes, Dorothea Duong, Vikram Chan-Herur, Anna Buchman, Marlene K Wolfe and Bradley J White

The Lancet Microbe, 2023, 4, e340
DOI: 10.1016/S2666-5247(22)00386-X

Detection of Hemagglutinin H5 Influenza A Virus Sequence in Municipal Wastewater Solids at Wastewater Treatment Plants with Increases in Influenza A in Spring, 2024

Marlene K. Wolfe, Dorothea Duong, Bridgette Shelden, Elana M. G. Chan, Vikram Chan-Herur, Stephen Hilton, Abigail Harvey Paulos, Xiang-Ru S. Xu, Alessandro Zulli, Bradley J. White and Alexandria B. Boehm

Environ. Sci. Technol. Lett., 2024, 11, 526
DOI: 10.1021/acs.estlett.4c00331

Research gaps and priorities for quantitative microbial risk assessment (QMRA)

Kerry A. Hamilton, Joanna Ciol Harrison, Jade Mitchell, Mark Weir, Marc Verhougstraete, Charles N. Haas, A. Pouyan Nejadhashemi, Julie Libarkin, Tiong Gim Aw, Kyle Bibby, Aaron Bivins, Joe Brown, Kara Dean, Gwyneth Dunbar, Joseph N. S. Eisenberg, Monica Emelko, Daniel Gerrity, Patrick L. Gurian, Emma Hartnett, Michael Jahne, Rachael M. Jones, Timothy R. Julian, Hongwan Li, Yanbin Li, Jacqueline MacDonald Gibson, Gertjan Medema, J. Scott Meschke, Alexis Mraz, Heather Murphy, David Oryang, Emmanuel de‐Graft Johnson Owusu‐Ansah, Emily Pasek, Abani K. Pradhan, Maria Tereza Pepe Razzolini, Michael O. Ryan, Mary Schoen, Patrick W. M. H. Smeets, Jeffrey Soller, Helena Solo‐Gabriele, Clinton Williams, Amanda M. Wilson, Amy Zimmer‐Faust, Jumana Alja'fari and Joan B. Rose

Risk Analysis, 2024, 44, 2521
DOI: 10.1111/risa.14318

Assessment of Public Health Agency and Utility Training Needs for CDC National Wastewater Surveillance System Jurisdictions in the United States

Rebecca Schneider, Kirsten Weisbeck, Komal Sheth, Porter Sikes, Kaavya Domakonda, Lauren Stadler, Katherine B. Ensor, Rachel Shaw, Cody Berkobien, Allison Wheeler, Catherine D. Johnson, Corinne Lengsfeld and Loren Hopkins

Health Promotion Practice, 2025, 26, 630
DOI: 10.1177/15248399241275617

Divergence of wastewater SARS-CoV-2 and reported laboratory-confirmed COVID-19 incident case data coincident with wide-spread availability of at-home COVID-19 antigen tests

Alexandria B. Boehm, Marlene K. Wolfe, Bradley White, Bridgette Hughes and Dorothea Duong

PeerJ, 2023, 11, e15631
DOI: 10.7717/peerj.15631

Persistence of human respiratory viral RNA in wastewater-settled solids

Mengyang Zhang, Laura Roldan-Hernandez, Alexandria Boehm and Christopher A. Elkins

Appl Environ Microbiol, 2024, 90
DOI: 10.1128/aem.02272-23