Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs

Jiabin Zhang, Seonho Yun, Jingxiu Bi, Sheng Dai, Yuguang Du, Andrew C. W. Zannettino and Hu Zhang

J. Mater. Chem. B, 2018, 6, 1799
DOI: 10.1039/C8TB00376A

Laser-textured surface storing a carbon dots/poly(ethylene glycol)/chitosan gel with slow-release lubrication effect

Hailin Lu, Shanshan Ren, Pengpeng Zhang, Junde Guo, Jianhui Li and Guangneng Dong

RSC Adv., 2017, 7, 21600
DOI: 10.1039/C7RA02387A

Mesenchymal stem cells loaded on 3D-printed gradient poly(ε-caprolactone)/methacrylated alginate composite scaffolds for cartilage tissue engineering

Yanyan Cao, Peng Cheng, Shengbo Sang, Chuan Xiang, Yang An, Xiaochun Wei, Zhizhong Shen, Yixia Zhang and Pengcui Li

Regenerative Biomaterials, 2021, 8
DOI: 10.1093/rb/rbab019

Massive fabrication of functional hepatic cancer spheroids by micropatterned GelMA hydrogel chip for drug screening

Fang Chen, Xiaoyun Wei, Keke Chen, Ling Wang and Mingen Xu

Colloids and Surfaces B: Biointerfaces, 2024, 244, 114171
DOI: 10.1016/j.colsurfb.2024.114171

Micro Pattern‐Based 3D Cell Culture Platform: An Overview of Technologies and Applications

Xinglong Zhu, Yi Li, Hulin Long, Yijia Wu, Ningwei Sun, Shun Li, Yiyao Liu, Huiqi Xie and Ji Bao

Exploration, 2026, 6
DOI: 10.1002/EXP.20240469

Three‐dimensional cell culture models for anticancer drug screening: Worth the effort?

Eddy‐Tim Verjans, Jordi Doijen, Walter Luyten, Bart Landuyt and Liliane Schoofs

Journal Cellular Physiology, 2018, 233, 2993
DOI: 10.1002/jcp.26052

High-throughput approaches for screening and analysis of cell behaviors

Jungmok Seo, Jung-Youn Shin, Jeroen Leijten, Oju Jeon, Gulden Camci-Unal, Anna D. Dikina, Katelyn Brinegar, Amir M. Ghaemmaghami, Eben Alsberg and Ali Khademhosseini

Biomaterials, 2018, 153, 85
DOI: 10.1016/j.biomaterials.2017.06.022

Applications and Advances of Multicellular Tumor Spheroids: Challenges in Their Development and Analysis

Achilleas G. Mitrakas, Avgi Tsolou, Stylianos Didaskalou, Lito Karkaletsou, Christos Efstathiou, Evgenios Eftalitsidis, Konstantinos Marmanis and Maria Koffa

IJMS, 2023, 24, 6949
DOI: 10.3390/ijms24086949

Progress of Degradable Synthetic Polymers in Adipose Tissue Engineering-A Review

Zhangsong Peng, Jie Chen, Yan Chen, Yuxin Chen, Yuheng Wang and Chao Han

j biomed nanotechnol, 2024, 20, 415
DOI: 10.1166/jbn.2024.3798

Smart alginate inks for tissue engineering applications

Mozhgan Keshavarz, Mohammadjavad Jahanshahi, Masoud Hasany, Firoz Babu Kadumudi, Mehdi Mehrali, Mohammad-Ali Shahbazi, Parvin Alizadeh, Gorka Orive and Alireza Dolatshahi-Pirouz

Materials Today Bio, 2023, 23, 100829
DOI: 10.1016/j.mtbio.2023.100829

In vitro construction of lung cancer organoids by 3D bioprinting for drug evaluation

Qianqian Dong, Xin Su, Xin Li, Huan Zhou, Honglei Jian, Shuo Bai, Jian Yin and Qingjun You

Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2023, 666, 131288
DOI: 10.1016/j.colsurfa.2023.131288

Physicochemical properties of ionic and non-ionic biocompatible hydrogels in water and cell culture conditions: Relation with type of morphologies of bovine fetal fibroblasts in contact with the surfaces

Rebeca Rivero, Fabrisio Alustiza, Virginia Capella, Cecilia Liaudat, Nancy Rodriguez, Pablo Bosch, Cesar Barbero and Claudia Rivarola

Colloids and Surfaces B: Biointerfaces, 2017, 158, 488
DOI: 10.1016/j.colsurfb.2017.07.032

Hydrogel microwells with customizable bottom design: A one‐step approach to spheroid formation

Ahmad A. Manzoor, Omar M. Rahman, Roberto Tarantino, Peter Zastawny, Stephen D. Waldman and Dae Kun Hwang

Can J Chem Eng, 2025, 103, 4647
DOI: 10.1002/cjce.25674

Architecture of an All‐In‐One Microfluidic Platform for Accelerated Cancer Seeding, Enhanced Spheroid Formation, and Dynamic Drug Screening Trials

Omar M. Rahman, Shengxi Lan and Dae Kun Hwang

Adv Materials Technologies, 2026, 11
DOI: 10.1002/admt.202501747

Rapid fabrication of sieved microwells and cross-flow microparticle trapping

Lauren Romita, Shyan Thompson and Dae Kun Hwang

Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-72700-5

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

Honglin Shen, Shuxiang Cai, Chuanxiang Wu, Wenguang Yang, Haibo Yu and Lianqing Liu

Micromachines, 2021, 12, 96
DOI: 10.3390/mi12010096

Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids

Se-jeong Kim, Jaesung Park, Hayeon Byun, Young-Woo Park, Luke G. Major, Dong Yun Lee, Yu Suk Choi and Heungsoo Shin

Biomaterials, 2019, 188, 198
DOI: 10.1016/j.biomaterials.2018.10.025

Self-Assemblable Polymer Smart-Blocks for Temperature-Induced Injectable Hydrogel in Biomedical Applications

Thai Thanh Hoang Thi, Le Hoang Sinh, Dai Phu Huynh, Dai Hai Nguyen and Cong Huynh

Front. Chem., 2020, 8
DOI: 10.3389/fchem.2020.00019

Hydrogel-based preparation of cell aggregates for biomedical applications

Jiabin Zhang, Seonho Yun, Yuguang Du, Andrew Zannettino and Hu Zhang

Applied Materials Today, 2020, 20, 100747
DOI: 10.1016/j.apmt.2020.100747

Oxidized Alginate-Based Hydrogels for Tissue Engineering Applications: A Review

Supachai Reakasame and Aldo R. Boccaccini

Biomacromolecules, 2018, 19, 3
DOI: 10.1021/acs.biomac.7b01331

Comparison of the formation, adipogenic maturation, and retention of human adipose‐derived stem cell spheroids in scaffold‐free culture techniques

Sarah J. Fitzgerald, Jared S. Cobb and Amol V. Janorkar

J Biomed Mater Res, 2020, 108, 3022
DOI: 10.1002/jbm.b.34631

Polymer Design of Microwell Hydrogels Influences Epithelial–Mesenchymal Interactions During Human Bronchosphere Formation

Madeline K. Eiken, Justin E. Levine, Shinyeong Lee, Samantha Lukpat, Eleanor M. Plaster, Vikram Bala, Jason R. Spence and Claudia Loebel

Advanced NanoBiomed Research, 2025, 5
DOI: 10.1002/anbr.202300110

Vascularized adipose tissue engineering: moving towards soft tissue reconstruction

Arne Peirsman, Huu Tuan Nguyen, Michiel Van Waeyenberge, Carlos Ceballos, Johana Bolivar, Satoru Kawakita, Florian Vanlauwe, Zuzana Tirpáková, Sofie Van Dorpe, Lana Van Damme, Marvin Mecwan, Menekse Ermis, Surjendu Maity, Kalpana Mandal, Rondinelli Herculano, Bernard Depypere, Lore Budiharto, Sandra Van Vlierberghe, Olivier De Wever, Phillip Blondeel, Vadim Jucaud, Mehmet Remzi Dokmeci and Ali Khademhosseini

Biofabrication, 2023, 15, 032003
DOI: 10.1088/1758-5090/acd7a5

Synthesis, properties, and biomedical applications of alginate methacrylate (ALMA)-based hydrogels: Current advances and challenges

Masoud Hasany, Sepehr Talebian, Seyedmostafa Sadat, Navid Ranjbar, Mohammad Mehrali, Gordon G. Wallace and Mehdi Mehrali

Applied Materials Today, 2021, 24, 101150
DOI: 10.1016/j.apmt.2021.101150

Jammed Micro‐Flake Hydrogel for Four‐Dimensional Living Cell Bioprinting

Aixiang Ding, Oju Jeon, David Cleveland, Kaelyn L. Gasvoda, Derrick Wells, Sang Jin Lee and Eben Alsberg

Advanced Materials, 2022, 34
DOI: 10.1002/adma.202109394

Automated Assessment of Cancer Drug Efficacy On Breast Tumor Spheroids in Aggrewell™400 Plates Using Image Cytometry

Shilpaa Mukundan, Jordan Bell, Matthew Teryek, Charles Hernandez, Andrea C. Love, Biju Parekkadan and Leo Li-Ying Chan

J Fluoresc, 2022, 32, 521
DOI: 10.1007/s10895-021-02881-3

Fabrication approaches for high-throughput and biomimetic disease modeling

Mackenzie L. Grubb and Steven R. Caliari

Acta Biomaterialia, 2021, 132, 52
DOI: 10.1016/j.actbio.2021.03.006

Single-Step Fabrication of V-Shaped Polymeric Microwells to Enhance Cancer Spheroid Formation

Omar M. Rahman, Roberto Tarantino, Stephen D. Waldman and Dae Kun Hwang

ACS Biomater. Sci. Eng., 2025, 11, 1857
DOI: 10.1021/acsbiomaterials.4c02359

Thermoresponsive poly(N-isopropylacrylamide) hydrogel substrates micropatterned with poly(ethylene glycol) hydrogel for adipose mesenchymal stem cell spheroid formation and retrieval

Gyeongju Kim, Yuna Jung, Kanghee Cho, Hyun Jong Lee and Won-Gun Koh

Materials Science and Engineering: C, 2020, 115, 111128
DOI: 10.1016/j.msec.2020.111128

3D Cell Culture Systems: Tumor Application, Advantages, and Disadvantages

Ola Habanjar, Mona Diab-Assaf, Florence Caldefie-Chezet and Laetitia Delort

IJMS, 2021, 22, 12200
DOI: 10.3390/ijms222212200

Human Adipose-Derived Stromal/Stem Cell Culture and Analysis Methods for Adipose Tissue Modeling In Vitro: A Systematic Review

Peyton Gibler, Jeffrey Gimble, Katie Hamel, Emma Rogers, Michael Henderson, Xiying Wu, Spencer Olesky and Trivia Frazier

Cells, 2021, 10, 1378
DOI: 10.3390/cells10061378

Self‐Healing Gelatin Hydrogels Cross‐Linked by Combining Multiple Hydrogen Bonding and Ionic Coordination

Guangzhao Zhang, Lei Lv, Yonghong Deng and Chaoyang Wang

Macromol. Rapid Commun., 2017, 38
DOI: 10.1002/marc.201700018

3D Bioprinting a human iPSC-derived MSC-loaded scaffold for repair of the uterine endometrium

Wanqing Ji, Bo Hou, Weige Lin, Linli Wang, Wenhan Zheng, Weidong Li, Jie Zheng, Xuejun Wen and Ping He

Acta Biomaterialia, 2020, 116, 268
DOI: 10.1016/j.actbio.2020.09.012

Photocrosslinkable Silk-Based Biomaterials for Regenerative Medicine and Healthcare Applications

Souradeep Dey, Chitra Jaiswal, Sayanti Shome, Bibrita Bhar, Ashutosh Bandyopadhyay, Kodieswaran Manikumar, Rajat Dadheech and Biman B. Mandal

Regen. Eng. Transl. Med., 2023, 9, 181
DOI: 10.1007/s40883-022-00277-8