Cross flow coupled with inertial focusing for separation of human sperm cells from semen and simulated TESE samples

Amid Rahi, Mahsa Kazemi, Esmail Pishbin, Sareh Karimi and Hamid Nazarian

Analyst, 2021, 146, 7230
DOI: 10.1039/D1AN01525G

Virus removal from semen with a pinched flow fractionation microfluidic chip

T. Hamacher, J. T. W. Berendsen, J. E. van Dongen, R. M. van der Hee, J. J. L. M. Cornelissen, M. L. W. J. Broekhuijse and L. I. Segerink

Lab Chip, 2021, 21, 4477
DOI: 10.1039/D1LC00643F

Rapid separation of human breast cancer cells from blood using a simple spiral channel device

Di Huang, Xin Shi, Yi Qian, Wenlai Tang, Linbo Liu, Nan Xiang and Zhonghua Ni

Anal. Methods, 2016, 8, 5940
DOI: 10.1039/C6AY01077F

Shear-enhanced sorting of ovoid and filamentous bacterial cells using pinch flow fractionation

Guillaume de Timary, Clothilde J. Rousseau, Laurence Van Melderen and Benoit Scheid

Lab Chip, 2023, 23, 659
DOI: 10.1039/D2LC00969B

High efficiency rare sperm separation from biopsy samples in an inertial focusing device

Haidong Feng, Alex Jafek, Raheel Samuel, James Hotaling, Timothy G. Jenkins, Kenneth I. Aston and Bruce K. Gale

Analyst, 2021, 146, 3368
DOI: 10.1039/D1AN00480H

Deciphering the unique inertial focusing behavior of sperm cells

Mohammad Moein Naderi, Hua Gao, Jian Zhou, Ian Papautsky and Zhangli Peng

Lab Chip, 2025, 25, 2874
DOI: 10.1039/D5LC00047E

Super-enhanced particle focusing in a novel microchannel geometry using inertial microfluidics

U Sonmez, S Jaber and L Trabzon

J. Micromech. Microeng., 2017, 27, 065003
DOI: 10.1088/1361-6439/aa6b18

Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens

Raheel Samuel, Jiyoung Son, Timothy G. Jenkins, Alexander Jafek, Haidong Feng, Bruce K. Gale, Douglas T. Carrell and James M. Hotaling

Urology, 2020, 140, 70
DOI: 10.1016/j.urology.2019.12.053

A novel microfluidic device with parallel channels for sperm separation using spermatozoa intrinsic behaviors

Ali Heydari, Mohammad Zabetian Targhi, Iman Halvaei and Reza Nosrati

Sci Rep, 2023, 13
DOI: 10.1038/s41598-023-28315-7

Label-Free, High-Throughput Assay of Human Dendritic Cells from Whole-Blood Samples with Microfluidic Inertial Separation Suitable for Resource-Limited Manufacturing

Mohamed Yousuff Caffiyar, Kue Peng Lim, Ismail Hussain Kamal Basha, Nor Hisham Hamid, Sok Ching Cheong and Eric Tatt Wei Ho

Micromachines, 2020, 11, 514
DOI: 10.3390/mi11050514

Microfluidics: The future of sperm selection in assisted reproduction

Ali Reza Jahangiri, Niloofar Ziarati, Ehsan Dadkhah, Mustafa Numan Bucak, Pegah Rahimizadeh, Abdolhossein Shahverdi, Mohammad Ali Sadighi Gilani and Tohid Rezaei Topraggaleh

Andrology, 2024, 12, 1236
DOI: 10.1111/andr.13578

Effect of various parameters on the distribution and extraction of platelets in a microfluidic system

Vijai Laxmi, Suhas S. Joshi and Amit Agrawal

Microfluid Nanofluid, 2021, 25
DOI: 10.1007/s10404-021-02464-5

Lab-on-Chip Systems for Cell Sorting: Main Features and Advantages of Inertial Focusing in Spiral Microchannels

Isabella Petruzzellis, Rebeca Martínez Vázquez, Stefania Caragnano, Caterina Gaudiuso, Roberto Osellame, Antonio Ancona and Annalisa Volpe

Micromachines, 2024, 15, 1135
DOI: 10.3390/mi15091135

Continuous separation of bacterial cells from large debris using a spiral microfluidic device

Ayomikun Esan, Frédérique Vanholsbeeck, Simon Swift and Cushla M. McGoverin

Biomicrofluidics, 2023, 17
DOI: 10.1063/5.0159254

Advances in Technical Assessment of Spiral Inertial Microfluidic Devices Toward Bioparticle Separation and Profiling: A Critical Review

Mahsa Bagi, Fatemeh Amjad, Seyed Majid Ghoreishian, Somayeh Sohrabi Shahsavari, Yun Suk Huh, Mostafa Keshavarz Moraveji and Sirivatch Shimpalee

BioChip J, 2024, 18, 45
DOI: 10.1007/s13206-023-00131-1

Separation of sperm cells from samples containing high concentrations of white blood cells using a spiral channel

Jiyoung Son, Raheel Samuel, Bruce K. Gale, Douglas T. Carrell and James M. Hotaling

Biomicrofluidics, 2017, 11
DOI: 10.1063/1.4994548

An automated instrument for intrauterine insemination sperm preparation

Alex Jafek, Haidong Feng, Hayden Brady, Kevin Petersen, Marzieh Chaharlang, Kenneth Aston, Bruce Gale, Timothy Jenkins and Raheel Samuel

Sci Rep, 2020, 10
DOI: 10.1038/s41598-020-78390-3

Sorting algal cells by morphology in spiral microchannels using inertial microfluidics

Allison Schaap, Jéromine Dumon and Jaap den Toonder

Microfluid Nanofluid, 2016, 20
DOI: 10.1007/s10404-016-1787-1

Optimization of a microfluidic spiral channel used to separate sperm from blood cells

Sabin Nepal, Haidong Feng and Bruce K. Gale

Biomicrofluidics, 2020, 14, 064103
DOI: 10.1063/5.0029508

A microfluidic lab chip for the manipulation and co-culturing of embryos with stromal cells

Yu-Shih Chen, Tzu-Wei Lo, Hong-Yuan Huang, Lien-Min Li, Yi-Wen Wang, Da-Jeng Yao, Wen-Syang Hsu and Cheng-Hsien Liu

Sensors and Actuators B: Chemical, 2021, 349, 130820
DOI: 10.1016/j.snb.2021.130820

Microfluidics facilitating the use of small extracellular vesicles in innovative approaches to male infertility

Dale M. Goss, Steven A. Vasilescu, Gavin Sacks, David K. Gardner and Majid E. Warkiani

Nat Rev Urol, 2023, 20, 66
DOI: 10.1038/s41585-022-00660-8

Separation of mononuclear cells from progenitor products by a novel inertial microfluidic method

Nilgün Okşak, Sultan Sahin Keskin, Esin Cetin Aktas, Zeynep Dogusan, Levent Trabzon and Dürdane Serap Erdem Kuruca

Biomed Microdevices, 2025, 27
DOI: 10.1007/s10544-025-00756-z

A novel two-stage microfluidic channel for high-quality sperm separation and washing

Saeed Derakhshan, Ataallah Kamyabi, Sareh Ashourzadeh and Tooraj Reza Mirshekari

Appl. Phys. A, 2025, 131
DOI: 10.1007/s00339-025-08761-y

Detection of Rare Objects by Flow Cytometry: Imaging, Cell Sorting, and Deep Learning Approaches

Denis V. Voronin, Anastasiia A. Kozlova, Roman A. Verkhovskii, Alexey V. Ermakov, Mikhail A. Makarkin, Olga A. Inozemtseva and Daniil N. Bratashov

IJMS, 2020, 21, 2323
DOI: 10.3390/ijms21072323

A novel microfluidic system to separate sperm using spermatozoa inherent motion and inertial effect

Anahita Ghassemi Panah, Mohammad Zabetian Targhi, Ali Heydari and Iman Halvaei

Journal of Biomechanics, 2022, 142, 111256
DOI: 10.1016/j.jbiomech.2022.111256

Laboratory and clinical management of leukocytospermia and hematospermia: a review

Kajal Khodamoradi, Manish Kuchakulla, Manish Narasimman, Zahra Khosravizadeh, Aleena Ali, Nancy Brackett, Emad Ibrahim and Ranjith Ramasamy

Clin Med�Insights�Reprod�Health, 2020, 14
DOI: 10.1177/2633494120922511

Effect of microfluidic processing on the viability of boar and bull spermatozoa

Tanja Hamacher, Johanna T. W. Berendsen, Stella A. Kruit, Marleen L. W. J. Broekhuijse and Loes I. Segerink

Biomicrofluidics, 2020, 14
DOI: 10.1063/5.0013919

Multi-dimensional-double-spiral (MDDS) inertial microfluidic platform for sperm isolation directly from the raw semen sample

Hyungkook Jeon, Claudia Cremers, Doris Le, Justin Abell and Jongyoon Han

Sci Rep, 2022, 12
DOI: 10.1038/s41598-022-08042-1

Size and shape based chromosome separation in the inertial focusing device

Haidong Feng, Matthew Hockin, Mario Capecchi, Bruce Gale and Himanshu Sant

Biomicrofluidics, 2020, 14
DOI: 10.1063/5.0026281

Advances in Microfluidics‐Based Assisted Reproductive Technology: From Sperm Sorter to Reproductive System‐on‐a‐Chip

Navid Kashaninejad, Muhammad Johirul Alam Shiddiky and Nam‐Trung Nguyen

Adv. Biosys., 2018, 2
DOI: 10.1002/adbi.201700197

Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties

Caffiyar Yousuff, Eric Ho, Ismail Hussain K. and Nor Hamid

Micromachines, 2017, 8, 15
DOI: 10.3390/mi8010015

Sperm-like-particle (SLP) behavior in curved microfluidic channels

Jiyoung Son, Alexander R. Jafek, Douglas T. Carrell, James M. Hotaling and Bruce K. Gale

Microfluid Nanofluid, 2019, 23
DOI: 10.1007/s10404-018-2170-1

Optimization of Dean flow microfluidic chip for sperm preparation for intrauterine insemination

Alex Jafek, Haidong Feng, Dallin Broberg, Bruce Gale, Raheel Samuel, Kenneth Aston and Timothy Jenkins

Microfluid Nanofluid, 2020, 24
DOI: 10.1007/s10404-020-02366-y

Separation of spermatozoa from erythrocytes using their tumbling mechanism in a pinch flow fractionation device

Johanna T. W. Berendsen, Jan C. T. Eijkel, Alex M. Wetzels and Loes I. Segerink

Microsyst Nanoeng, 2019, 5
DOI: 10.1038/s41378-019-0068-z

A curved expansion-contraction microfluidic structure for inertial based separation of circulating tumor cells from blood samples

Sina Ebrahimi, Mojgan Alishiri, Esmail Pishbin, Homa Afjoul and Amir Shamloo

Journal of Chromatography A, 2023, 1705, 464200
DOI: 10.1016/j.chroma.2023.464200

Recent advances in sex-specific reproductive microphysiological systems for the systematic evaluation of assisted reproductive technology

Minkyeong Jeong, Kiyeon Park, Dawit Jung, Sieun Shin, Jungseub Lee, Jihoon Ko, Seokyoung Bang and Jungho Ahn

Materials Today Bio, 2026, 38, 103050
DOI: 10.1016/j.mtbio.2026.103050

Inertial Microfluidics Enabling Clinical Research

Srivathsan Kalyan, Corinna Torabi, Harrison Khoo, Hyun Woo Sung, Sung-Eun Choi, Wenzhao Wang, Benjamin Treutler, Dohyun Kim and Soojung Claire Hur

Micromachines, 2021, 12, 257
DOI: 10.3390/mi12030257

Passive microfluidic devices for cell separation

Tianlong Zhang, Dino Di Carlo, Chwee Teck Lim, Tianyuan Zhou, Guizhong Tian, Tao Tang, Amy Q. Shen, Weihua Li, Ming Li, Yang Yang, Keisuke Goda, Ruopeng Yan, Cheng Lei, Yoichiroh Hosokawa and Yaxiaer Yalikun

Biotechnology Advances, 2024, 71, 108317
DOI: 10.1016/j.biotechadv.2024.108317

Design, Fabrication, and Testing of a Microfluidic Device for Thermotaxis and Chemotaxis Assays of Sperm

Yong-Jun Ko, Joon-Ho Maeng, Seung Yong Hwang and Yoomin Ahn

SLAS Technology, 2018, 23, 507
DOI: 10.1177/2472630318783948

A microfluidic approach to rapid sperm recovery from heterogeneous cell suspensions

Steven A. Vasilescu, Shayan Khorsandi, Lin Ding, Sajad Razavi Bazaz, Reza Nosrati, Debra Gook and Majid Ebrahimi Warkiani

Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-87046-9

The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices

Nima Ahmadkhani, Mahshid Hosseini, Maryam Saadatmand and Alireza Abbaspourrad

J Assist Reprod Genet, 2022, 39, 19
DOI: 10.1007/s10815-021-02377-w

Experimental study on the effect of flow in microfluidic channel on bovine sperm navigation

Toru Hyakutake, Kenta Sugita, Shota Ujifuku, Rintaro Sakurai, Renta Murakami and Yasutaka Hayamizu

Journal of Biomechanics, 2021, 118, 110290
DOI: 10.1016/j.jbiomech.2021.110290

Separation of U87 glioblastoma cell-derived small and medium extracellular vesicles using elasto-inertial flow focusing (a spiral channel)

Farhad Shiri, Haidong Feng, Kevin E. Petersen, Himanshu Sant, Gina T. Bardi, Luke A. Schroeder, Michael L. Merchant, Bruce K. Gale and Joshua L. Hood

Sci Rep, 2022, 12
DOI: 10.1038/s41598-022-10129-8

Instrumentation for xPCR Incorporating qPCR and HRMA

Alexander R. Jafek, Sean Harbertson, Hayden Brady, Raheel Samuel and Bruce K. Gale

Anal. Chem., 2018, 90, 7190
DOI: 10.1021/acs.analchem.7b05176

Design clues for motility and rheotaxis-based microfluidic chips for sperm sorting

Olga Smirnova, Daniil Golubchikov, Anton Murashko, Nastasia Kosheleva, Maryam Saadatmand, Peter Timashev and Anastasia Shpichka

Prog. Biomed. Eng., 2025, 7, 042004
DOI: 10.1088/2516-1091/adf92f

Effects of Shear and Extensional Stresses on Cells: Investigation in a Spiral Microchannel and Contraction–Expansion Arrays

Thammawit Suwannaphan, Ampol Kamnerdsook, Suramate Chalermwisutkul, Boonchai Techaumnat, Nattapol Damrongplasit, Bhawat Traipattanakul, Surasak Kasetsirikul and Alongkorn Pimpin

ACS Biomater. Sci. Eng., 2025, 11, 3249
DOI: 10.1021/acsbiomaterials.5c00555

Separating mouse malignant cell line (EL4) from neonate spermatogonial stem cells utilizing microfluidic device in vitro

Behnaz Ashtari, Azar Shams, Narges Esmaeilzadeh, Sara Tanbakooei, Morteza Koruji, Mojtaba Johari Moghadam, Javad Mohajer Ansari, Adel Johari Moghadam and Ronak Shabani

Stem Cell Res Ther, 2020, 11
DOI: 10.1186/s13287-020-01671-1

Limitation of spiral microchannels for particle separation in heterogeneous mixtures: Impact of particles’ size and deformability

Ewa Guzniczak, Timm Krüger, Helen Bridle and Melanie Jimenez

Biomicrofluidics, 2020, 14
DOI: 10.1063/5.0009673

Single layer centrifugation combined with brief abstinence for semen cryopreservation in a patient with hematospermia: Case report and literature review

Yuan Liu, Yang Xian, Bo Liu, Wenrui Zhao, Lijuan Ying, Jinyan Xu, Xuefeng Luo, Chen Luo and Fuping Li

Medicine, 2025, 104, e43272
DOI: 10.1097/MD.0000000000043272

Prediction of sperm motion behavior in microfluidic channel using sperm swimming model

Toru Hyakutake, Daisuke Higashiyama and Tomoka Tsuchiya

Journal of Biomechanics, 2024, 176, 112336
DOI: 10.1016/j.jbiomech.2024.112336

IVF-on-a-Chip: Recent Advances in Microfluidics Technology for In Vitro Fertilization

Lindong Weng

SLAS Technology, 2019, 24, 373
DOI: 10.1177/2472630319851765

Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities

Giuseppina Marzano, Maria Serena Chiriacò, Elisabetta Primiceri, Maria Elena Dell’Aquila, João Ramalho-Santos, Vincenzo Zara, Alessandra Ferramosca and Giuseppe Maruccio

Biotechnology Advances, 2020, 40, 107498
DOI: 10.1016/j.biotechadv.2019.107498

Microfluidic innovations driving the future of sperm motility and sex sorting techniques

Vidhi Das, Abinaya Rajendran, Lakshmi Krishnan, Fan-Gang Tseng and Tuhin Subhra Santra

Biomaterials Advances, 2026, 214867
DOI: 10.1016/j.bioadv.2026.214867