Issue 79, 2012
From the journal:

Chemical Communications

Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides†

Vanessa Siegmund,a   Tobias Santner,b   Ronald Micurab  and  Andreas Marx*a  

* Corresponding authors

a Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany
E-mail: andreas.marx@uni-konstanz.de
Fax: +49 7531-885140
Tel: +49 7531-885139

b Institute of Organic Chemistry, Center for Chemistry and Biomedicine, Leopold-Franzens University, Innrain 80-82, 6020 Innsbruck, Austria

Abstract

We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2′-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2′-methylseleno-2′-deoxyuridine 5′-triphosphate.

Graphical abstract: Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides

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Article information

DOI
https://doi.org/10.1039/C2CC35028A
Article type
Communication
Submitted
13 Jul 2012
Accepted
13 Aug 2012
First published
16 Aug 2012

Chem. Commun., 2012,48, 9870-9872

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Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2′-modified nucleotides

V. Siegmund, T. Santner, R. Micura and A. Marx, Chem. Commun., 2012, 48, 9870 DOI: 10.1039/C2CC35028A

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