Photoinitiated thiol–ene mediated functionalisation of 4,5-enoses

Alejandro Prieto-Castañeda, Harlei Martin, Tapasi Manna, Laura Beswick, Joshua T. McLean, Imlirenla Pongener, Inés Rabadán González, Brendan Twamley, Gavin J. Miller and Eoin M. Scanlan

Org. Biomol. Chem., 2025, 23, 5332
DOI: 10.1039/D5OB00507H

Phosphorus sustainability: a case for phytic acid as a biorenewable platform

Emma K. Davison, Jessica C. Neville and Jonathan Sperry

Green Chem., 2023, 25, 5390
DOI: 10.1039/D3GC01421E

Efficient chemoenzymatic synthesis of novel galacto-N-biose derivatives and their sialylated forms

Lei Li, Yonghui Liu, Tiehai Li, Wenjun Wang, Zaikuan Yu, Cheng Ma, Jingyao Qu, Wei Zhao, Xi Chen and Peng G. Wang

Chem. Commun., 2015, 51, 10310
DOI: 10.1039/C5CC03746H

One-pot multienzyme (OPME) systems for chemoenzymatic synthesis of carbohydrates

Hai Yu and Xi Chen

Org. Biomol. Chem., 2016, 14, 2809
DOI: 10.1039/C6OB00058D

Enzyme and microbial technology for synthesis of bioactive oligosaccharides: an update

Rachel Chen

Appl Microbiol Biotechnol, 2018, 102, 3017
DOI: 10.1007/s00253-018-8839-2

Protein Fusion of Biosynthetic Enzymes and a Thermo‐Responsive Polypeptide Expedites Facile Access to Biocatalysts for Nucleotide Sugars

Xiaocong Wu, Jing Liu, Xuefei Yin, Di Ma, Sichao Zhang and Xianwei Liu

ChemBioChem, 2025, 26
DOI: 10.1002/cbic.202401005

Facile and Stereo-Selective Synthesis of UDP-α-D-xylose and UDP-β-L-arabinose Using UDP-Sugar Pyrophosphorylase

JiaJia Wang, Harmon Greenway, Shanshan Li, Mohui Wei, Samuel J. Polizzi and Peng G. Wang

Front. Chem., 2018, 6
DOI: 10.3389/fchem.2018.00163

Systematic Enzymatic Synthesis of dTDP‐Activated Sugar Nucleotides

Fangyu Wei, Rui Yuan, Qin Wen and Liuqing Wen

Angew Chem Int Ed, 2023, 62
DOI: 10.1002/anie.202217894

Current state on the enzymatic synthesis of glycosaminoglycans

Johannes Gottschalk and Lothar Elling

Current Opinion in Chemical Biology, 2021, 61, 71
DOI: 10.1016/j.cbpa.2020.09.008

Biological strategies for oligo/polysaccharide synthesis: biocatalyst and microbial cell factory

Liting Zhao, Zhongbao Ma, Jian Yin, Guiyang Shi and Zhongyang Ding

Carbohydrate Polymers, 2021, 258, 117695
DOI: 10.1016/j.carbpol.2021.117695

Simultaneous one-pot multienzymatic synthesis and purification of Uridine-5′-Diphosphate-α-D-glucuronide and Uridine-5′-Diphosphate-α-d-xylose

Arnaud Pâris, Tiffany Rundstadler and Pierre Lafite

Carbohydrate Research, 2025, 554, 109516
DOI: 10.1016/j.carres.2025.109516

The Coupling of a Universal Polyphosphate Kinase and Glycosyltransferases Enabled In Situ Regeneration of Sugar Nucleotides for Efficient Synthesis of Functional Glycans

Xiaocong Wu, Haodong Wu, Yida Gao, Guofeng Gu and Xianwei Liu

ACS Catal., 2025, 15, 20745
DOI: 10.1021/acscatal.5c07552

Chemoenzymatic Synthesis of O-Mannose Glycans Containing Sulfated or Nonsulfated HNK-1 Epitope

Tian Gao, Jingyu Yan, Chang-Cheng Liu, Angelina S. Palma, Zhimou Guo, Min Xiao, Xi Chen, Xinmiao Liang, Wengang Chai and Hongzhi Cao

J. Am. Chem. Soc., 2019, 141, 19351
DOI: 10.1021/jacs.9b08964

Recent advances in the enzymatic synthesis of sugar-nucleotides using nucleotidylyltransferases and glycosyltransferases

Sanaz Ahmadipour, Laura Beswick and Gavin J. Miller

Carbohydrate Research, 2018, 469, 38
DOI: 10.1016/j.carres.2018.09.002

Efficient One-Pot Multienzyme Synthesis of β-Glycosides

Xiao-Lin Meng, Jian-Qun Deng, Jin-Qi Yu, Yi-Jing Chen, Yue-Ting Peng, Mei-Xin Xu, Feng-Shan Wang, Feng Geng, De-Qing Wang and Ju-Zheng Sheng

J. Agric. Food Chem., 2025, 73, 27611
DOI: 10.1021/acs.jafc.5c06416

Toward Automated Enzymatic Synthesis of Oligosaccharides

Liuqing Wen, Garrett Edmunds, Christopher Gibbons, Jiabin Zhang, Madhusudhan Reddy Gadi, Hailiang Zhu, Junqiang Fang, Xianwei Liu, Yun Kong and Peng George Wang

Chem. Rev., 2018, 118, 8151
DOI: 10.1021/acs.chemrev.8b00066

New Means to Control Molecular Assembly

Jiali Zhang, Hai Yu, Bradley Harris, Yunbo Zheng, Umit Celik, Lan Na, Roland Faller, Xi Chen, Dominik R. Haudenschild and Gang-yu Liu

J. Phys. Chem. C, 2020, 124, 6405
DOI: 10.1021/acs.jpcc.9b11377

Exploring Cell-Free Systems for Enhanced Synthesis of Hyaluronic Acid

Zhi-Yuan Yao, Jin-Song Gong, Xiongyan Yang, Jiancheng Shen, Min-Jun Yu, Chang Su, Heng Li, Guoqiang Xu, Shouyong Jiang, Frank Hollmann, Zheng-Hong Xu and Jin-Song Shi

ACS Sustainable Chem. Eng., 2025, 13, 20194
DOI: 10.1021/acssuschemeng.5c08932

A modular chemoenzymatic cascade strategy for the structure-customized assembly of ganglioside analogs

Xuefeng Jin, Hanchao Cheng, Xiaohui Chen, Xuefeng Cao, Cong Xiao, Fengling Ding, Huirong Qu, Peng George Wang, Yan Feng and Guang-Yu Yang

Commun Chem, 2024, 7
DOI: 10.1038/s42004-024-01102-9

Overview of the current procedures in synthesis of heparin saccharides

Siran Zhao, Tianji Zhang, Ying Kan, Hongmei Li and Jin-ping Li

Carbohydrate Polymers, 2024, 339, 122220
DOI: 10.1016/j.carbpol.2024.122220

The characterisation of a galactokinase from Streptomyces coelicolor

Tessa Keenan, Rhys Mills, Emily Pocock, Darshita Budhadev, Fabio Parmeggiani, Sabine Flitsch and Martin Fascione

Carbohydrate Research, 2019, 472, 132
DOI: 10.1016/j.carres.2018.12.005

Cell-Free Synthetic Glycobiology: Designing and Engineering Glycomolecules Outside of Living Cells

Thapakorn Jaroentomeechai, May N. Taw, Mingji Li, Alicia Aquino, Ninad Agashe, Sean Chung, Michael C. Jewett and Matthew P. DeLisa

Front. Chem., 2020, 8
DOI: 10.3389/fchem.2020.00645

Leloir Glycosyltransferases in Applied Biocatalysis: A Multidisciplinary Approach

Luuk Mestrom, Marta Przypis, Daria Kowalczykiewicz, André Pollender, Antje Kumpf, Stefan R. Marsden, Isabel Bento, Andrzej B. Jarzębski, Katarzyna Szymańska, Arkadiusz Chruściel, Dirk Tischler, Rob Schoevaart, Ulf Hanefeld and Peter-Leon Hagedoorn

IJMS, 2019, 20, 5263
DOI: 10.3390/ijms20215263

Thermophilic Inorganic Pyrophosphatase Ton1914 from Thermococcus onnurineus NA1 Removes the Inhibitory Effect of Pyrophosphate

Yajing Li, Xue Yang and Renjun Gao

IJMS, 2022, 23, 12735
DOI: 10.3390/ijms232112735

Multi-enzyme systems and recombinant cells for synthesis of valuable saccharides: Advances and perspectives

Jiangang Yang, Tong Zhang, Chaoyu Tian, Yueming Zhu, Yan Zeng, Yan Men, Peng Chen, Yuanxia Sun and Yanhe Ma

Biotechnology Advances, 2019, 37, 107406
DOI: 10.1016/j.biotechadv.2019.06.005

Engineer P. multocida Heparosan Synthase 2 (PmHS2) for Size-Controlled Synthesis of Longer Heparosan Oligosaccharides

Lan Na, Hai Yu, John B. McArthur, Tamashree Ghosh, Thomas Asbell and Xi Chen

ACS Catal., 2020, 10, 6113
DOI: 10.1021/acscatal.0c01231

Construction of immobilized enzyme cascades for the biosynthesis of nucleotide sugars UDP-N-acetylglucosamine and UDP-glucuronic acid

Jialian Li, Yanlai Liu, Litao Hu, Ruirui Xu, Weijiao Zhang, Shan Hu, Yang Wang, Guocheng Du and Zhen Kang

Syst Microbiol and Biomanuf, 2024, 4, 895
DOI: 10.1007/s43393-024-00238-y

Galactokinase-like protein from Leishmania donovani: Biochemical and structural characterization of a recombinant protein

Hasana Baber, Arega Aghajani, B. Harold Gallimore, Cassandra Bethel, James G. Hyatt, Elizabeth F.B. King, Helen P. Price, Marissa L. Maciej-Hulme, Suat Sari and Anja Winter

Biochimie, 2024, 223, 31
DOI: 10.1016/j.biochi.2024.03.017

Methods for the synthesis of natural UDP-sugars and synthetic analogues

Smritilekha Bera

Carbohydrate Research, 2025, 558, 109651
DOI: 10.1016/j.carres.2025.109651

Chemical and enzymatic synthesis of the alginate sugar nucleotide building block: GDP-d-mannuronic acid

Laura Beswick, Sanaz Ahmadipour, Jonathan P. Dolan, Martin Rejzek, Robert A. Field and Gavin J. Miller

Carbohydrate Research, 2019, 485, 107819
DOI: 10.1016/j.carres.2019.107819

Nucleotide Sugars in Chemistry and Biology

Satu Mikkola

Molecules, 2020, 25, 5755
DOI: 10.3390/molecules25235755

Synthesis of α-Deoxymono and Difluorohexopyranosyl 1-Phosphates and Kinetic Evaluation with Thymidylyl- and Guanidylyltransferases

Jian-She Zhu, Nicole E. McCormick, Shannon C. Timmons and David L. Jakeman

J. Org. Chem., 2016, 81, 8816
DOI: 10.1021/acs.joc.6b01485

Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis

L. X. Zang, R. R. Du, H. C. Zang, F. S. Wang and J. Z. Sheng

Appl Biochem Microbiol, 2019, 55, 631
DOI: 10.1134/S0003683819060152

Donor substrate promiscuity of bacterial β1–3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1–4-galactosyltransferases

Yanhong Li, Mengyang Xue, Xue Sheng, Hai Yu, Jie Zeng, Vireak Thon, Yi Chen, Musleh M. Muthana, Peng G. Wang and Xi Chen

Bioorganic & Medicinal Chemistry, 2016, 24, 1696
DOI: 10.1016/j.bmc.2016.02.043

Efficient chemoenzymatic synthesis of UDP-α-6-N3-glucose

Jiajia Wang, Dongzhe Zhang, Yinhang Wen, Xuefeng Cao, Jing Ma and Peng George Wang

Bioorganic & Medicinal Chemistry Letters, 2019, 29, 1148
DOI: 10.1016/j.bmcl.2019.02.002

Key Factors for a One-Pot Enzyme Cascade Synthesis of High Molecular Weight Hyaluronic Acid

Johannes Gottschalk, Henning Zaun, Anna Eisele, Jürgen Kuballa and Lothar Elling

IJMS, 2019, 20, 5664
DOI: 10.3390/ijms20225664

Cascade synthesis of uridine-5′-diphosphate glucuronic acid by coupling multiple whole cells expressing hyperthermophilic enzymes

Dan-Hua Meng, Ran-Ran Du, Lu-Zhou Chen, Meng-Ting Li, Fei Liu, Jin Hou, Yi-Kang Shi, Feng-Shan Wang and Ju-Zheng Sheng

Microb Cell Fact, 2019, 18
DOI: 10.1186/s12934-019-1168-z

Facile Synthesis of Sugar Nucleotides from Common Sugars by the Cascade Conversion Strategy

Shasha Wang, Jiabin Zhang, Fangyu Wei, Wanjin Li and Liuqing Wen

J. Am. Chem. Soc., 2022, 144, 9980
DOI: 10.1021/jacs.2c03138

Potential applications of recombinant bifidobacterial proteins in the food industry, biomedicine, process innovation and glycobiology

José A. Morales-Contreras, Jessica E. Rodríguez-Pérez, Carlos A. Álvarez-González, Mirian C. Martínez-López, Isela E. Juárez-Rojop and Ángela Ávila-Fernández

Food Sci Biotechnol, 2021, 30, 1277
DOI: 10.1007/s10068-021-00957-1

Concise synthesis of NDP-activated uronic acid by an oxidation reaction insertion strategy

Nana Yang, Rui Yuan, Xinyue Fu, Xiao Tian, Jin Yu, Shengzhou Ma, Liuqing Wen and Jiabin Zhang

Chinese Chemical Letters, 2025, 36, 110757
DOI: 10.1016/j.cclet.2024.110757

Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine

Yang Xu, Víctor M. Hernández-Rocamora, Joseph H. Lorent, Ruud Cox, Xiaoqi Wang, Xue Bao, Marjon Stel, Gaël Vos, Ramon M. van den Bos, Roland J. Pieters, Joe Gray, Waldemar Vollmer and Eefjan Breukink

iScience, 2022, 25, 104753
DOI: 10.1016/j.isci.2022.104753

Systematic Enzymatic Synthesis of dTDP‐Activated Sugar Nucleotides

Fangyu Wei, Rui Yuan, Qin Wen and Liuqing Wen

Angewandte Chemie, 2023, 135
DOI: 10.1002/ange.202217894

Strategies for Automated Enzymatic Glycan Synthesis (AEGS)

Kai Philip Hussnaetter, Philip Palm, Andrij Pich, Matthias Franzreb, Erdmann Rapp and Lothar Elling

Biotechnology Advances, 2023, 67, 108208
DOI: 10.1016/j.biotechadv.2023.108208