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This communication reports a new nanocatalytic scheme based on the facts that the redox reaction between a highly outer-sphere-reaction-philic (OSR-philic) species and a highly inner-sphere-reaction-philic (ISR-philic) species is slow and that an OSR- and ISR-philic Au-nanocatalyst label can mediate the two different types of redox species. This scheme allows highly sensitive and incubation free detection of creatine kinase-MB.

Graphical abstract: An ultrasensitive and incubation-free electrochemical immunosensor using a gold-nanocatalyst label mediating outer-sphere-reaction-philic and inner-sphere-reaction-philic species

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