Issue 20, 2021

Analysis of antioxidants in Chrysanthemum indici flos by online gradient extraction and HPLC-FRAP

Abstract

Chrysanthemum indici flos (CIF), a traditional functional beverage, has been used in China for thousands of years. However, research on the antioxidant properties and compounds in CIF is insufficient. In the current study, an online gradient extraction (OGE) coupled with a high performance liquid chromatography-ferric reducing antioxidant power (HPLC-FRAP) system for rapid identification of antioxidants in CIF was proposed. The CIF sample (0.3 mg) was extracted online by a gradient mobile phase (acetonitrile and 0.1% formic acid), separated on a Poroshell 120 SB-Aq column, and then detected for the anti-oxidative activity with the online FRAP assay. 36 peaks were found and 28 compounds were identified. Among them, 18 peaks showed antioxidant activity, six of which were detected in CIF for the first time. The total analytical time was less than 45 min, including sample extraction, separation, and antioxidant identification. The results indicate that the OGE-HPLC-FRAP system is a simple, efficient and rapid tool for screening antioxidants in CIF, which provides the scientific data of CIF's antioxidant research and is helpful to improve the quality evaluation of CIF.

Graphical abstract: Analysis of antioxidants in Chrysanthemum indici flos by online gradient extraction and HPLC-FRAP

Article information

Article type
Communication
Submitted
01 avr. 2021
Accepted
16 avr. 2021
First published
16 avr. 2021

Anal. Methods, 2021,13, 2283-2289

Analysis of antioxidants in Chrysanthemum indici flos by online gradient extraction and HPLC-FRAP

W. Fan, J. Zhou, Z. Wu, G. Tan, H. Li, Q. Mei and Z. Qian, Anal. Methods, 2021, 13, 2283 DOI: 10.1039/D1AY00548K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements