Issue 41, 2022

Development and validation of the UPLC-MS method for simultaneous determination of six new psychoactive substances

Abstract

The combined abuse of benzodiazepines and antipsychotics has become a global problem, and to develop a highly sensitive and selective method for monitoring of benzodiazepine hypnotics and antipsychotics is urgently necessary. In this work, we established a rapid method for the simultaneous determination of benzodiazepines (diazepam, alprazolam, triazolam, and estazolam) and antipsychotic drugs (clozapine, and chlorpromazine) based on ultra performance liquid chromatography-mass spectrometry (UPLC-MS). The accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), specificity, matrix effect and carry-over effect were verified in detail. The results of the recovery and repeat experiments proved that the proposed UPLC-MS method possessed very satisfactory accuracy and precision. The LOD and LOQ of the six psychoactive substances were as low as 0.001–0.005 and 0.005–0.01 μg L−1, respectively. The proposed method was employed to analyze urine samples which were pretreated with a protein precipitation process. The potential influences of precipitants on the analysis results were evaluated statistically, and 0.1% formic acid/acetonitrile/water was selected as the optimum precipitation agent. The detection of the targets was free from matrix and carryover effects.

Graphical abstract: Development and validation of the UPLC-MS method for simultaneous determination of six new psychoactive substances

Article information

Article type
Paper
Submitted
05 août 2022
Accepted
09 sept. 2022
First published
21 sept. 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 26704-26711

Development and validation of the UPLC-MS method for simultaneous determination of six new psychoactive substances

Z. Tan, Y. Wen, K. Yang, X. Zhao, X. Yang and J. He, RSC Adv., 2022, 12, 26704 DOI: 10.1039/D2RA04869H

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