Issue 14, 2022

Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration

Abstract

Heterogeneous immunoassays (HI) are an invaluable tool for biomarker detection and remain an ideal candidate for microfluidic point-of-care diagnostics. However, automating and controlling sustained fluid flow from benchtop to microfluidics for the HI reaction during the extended sample incubation step, remains difficult to implement; this leads to challenges for assay integration and assay result interpretation. To address these issues, we investigated the liquid reciprocation process on a microfluidic centrifugal disc (CD) to generate continuous, bidirectional fluid flow using only a rotating motor. Large volumetric flow rates (μL s−1) through the HI reaction chamber were sustained for extended durations (up to 1 h). The CD liquid reciprocation operating behavior was characterized experimentally and simulated to determine fluid flow shear rates through our HI reaction chamber. We demonstrated the continuous CD liquid reciprocation for target molecule incubation for a microarray HI and that higher fluid shear rates negatively influenced our fluorescence intensity. We highlight the importance of proper fluid flow considerations when integrating HIs with microfluidics.

Graphical abstract: Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration

Supplementary files

Article information

Article type
Paper
Submitted
05 mars 2022
Accepted
15 juin 2022
First published
21 juin 2022
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2022,22, 2695-2706

Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration

A. T. Hwu, M. Madadelahi, R. Nakajima, E. Shamloo, A. Perebikovsky, H. Kido, A. Jain, A. Jasinskas, S. Prange, P. Felgner and M. Madou, Lab Chip, 2022, 22, 2695 DOI: 10.1039/D2LC00213B

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