Issue 58, 2016

Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

Abstract

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

Graphical abstract: Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

Supplementary files

Article information

Article type
Communication
Submitted
18 janv. 2016
Accepted
31 mars 2016
First published
31 mars 2016
This article is Open Access
Creative Commons BY license

Chem. Commun., 2016,52, 9133-9136

Author version available

Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

L. A. Flanagan, J. J. Wright, M. M. Roessler, J. W. Moir and A. Parkin, Chem. Commun., 2016, 52, 9133 DOI: 10.1039/C6CC00515B

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