Issue 5, 2009

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

Abstract

We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow. Aqueous picoliter droplets are trapped en masse and optically monitored for extended periods of time. Such an array-based approach is used to characterize droplet shrinkage, aggregation of encapsulated E. colicells and enzymatic reactions. We also demonstrate that trapped droplets may be recovered from the microfluidic array for further processing.

Graphical abstract: Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

Supplementary files

Article information

Article type
Paper
Submitted
07 août 2008
Accepted
14 oct. 2008
First published
27 nov. 2008

Lab Chip, 2009,9, 692-698

Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

A. Huebner, D. Bratton, G. Whyte, M. Yang, A. J. deMello, C. Abell and F. Hollfelder, Lab Chip, 2009, 9, 692 DOI: 10.1039/B813709A

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