Issue 12, 2007

Quantitative detection of protein expression in single cells using droplet microfluidics

Abstract

We demonstrate that single cells can be controllably compartmentalized within aqueous microdroplets; using such an approach we perform high-throughput screening by detecting the expression of a fluorescent protein in individual cells with simultaneous measurement of droplet size and cell occupancy.

Graphical abstract: Quantitative detection of protein expression in single cells using droplet microfluidics

Supplementary files

Article information

Article type
Communication
Submitted
19 déc. 2006
Accepted
16 janv. 2007
First published
26 janv. 2007

Chem. Commun., 2007, 1218-1220

Quantitative detection of protein expression in single cells using droplet microfluidics

A. Huebner, M. Srisa-Art, D. Holt, C. Abell, F. Hollfelder, A. J. deMello and J. B. Edel, Chem. Commun., 2007, 1218 DOI: 10.1039/B618570C

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