Issue 14, 2021

Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2

Abstract

The COVID-19 pandemic, caused by severe acute respiratory coronavirus 2 (SARS-CoV-2), has become a public health emergency and widely spread around the world. Rapid, accurate and early diagnosis of COVID-19 infection plays a crucial role in breaking this pandemic. However, the detection accuracy is limited for current single-gene diagnosis of SARS-CoV-2. Herein, we develop an autonomous lab-on-paper platform for multiplex gene diagnosis of SARS-CoV-2 by combining reverse transcription recombinase polymerase amplification (RT-RPA) and CRISPR–Cas12a detection. The autonomous lab-on-paper is capable of simultaneously detecting nucleoprotein (N) gene and spike (S) gene of SARS-CoV-2 virus as well as human housekeeping RNAse P gene (an internal control) in a single clinical sample. With the developed platform, 102 copies viral RNA per test can be detected within one hour. Also, the lab-on-paper platform has been used to detect 21 swab clinical samples and obtains a comparable performance to the conventional RT-PCR method. Thus, the developed lab-on-paper platform holds great potential for rapid, sensitive, reliable, multiple molecular diagnostics of COVID-19 and other infectious diseases in resource-limited settings.

Graphical abstract: Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2

Supplementary files

Article information

Article type
Paper
Submitted
06 avr. 2021
Accepted
02 juin 2021
First published
02 juin 2021

Lab Chip, 2021,21, 2730-2737

Autonomous lab-on-paper for multiplexed, CRISPR-based diagnostics of SARS-CoV-2

K. Yin, X. Ding, Z. Li, M. M. Sfeir, E. Ballesteros and C. Liu, Lab Chip, 2021, 21, 2730 DOI: 10.1039/D1LC00293G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements