Issue 48, 2019

Peroxidase-AgAu hybrid nanocages as signal transducers for sensitive plasmonic colorimetric sensing

Abstract

In this work, we report a type of highly sensitive signal transducer – HRP-AgAu hybrid cages – for plasmonic colorimetric sensing which was constructed by functionalizing silver–gold nanocages (AgAu cages) with horseradish peroxidase (HRP). The high sensitivity of this signal transducer is ascribed to the fact that the immobilized HRP molecules on the AgAu cage surface can substantially promote the etching of Ag from the cages using H2O2 as an etchant, which induces sensitive multicolor changes. Compared to pristine AgAu cages, the HRP-AgAu hybrid cages were over 800 times more sensitive in response to H2O2. In addition to high sensitivity, the signal transducer features rapidity, simplicity, and visualization. Moreover, the signal transducer is compatible with the procedures and instruments of existing biosensing technologies, making it straightforward for practical applications. As a proof-of-concept demonstration, we applied the signal transducer in colorimetric sensing of glucose which showed a limit of detection as low as 0.5 μM.

Graphical abstract: Peroxidase-AgAu hybrid nanocages as signal transducers for sensitive plasmonic colorimetric sensing

Supplementary files

Article information

Article type
Paper
Submitted
09 sept. 2019
Accepted
15 oct. 2019
First published
17 oct. 2019

J. Mater. Chem. C, 2019,7, 15179-15187

Peroxidase-AgAu hybrid nanocages as signal transducers for sensitive plasmonic colorimetric sensing

Y. Wang, Z. Gao, B. Liu and X. Xia, J. Mater. Chem. C, 2019, 7, 15179 DOI: 10.1039/C9TC04966E

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