Themed collection Next wave advances in single cell analyses

Welcome to this Analyst themed issue highlighting next wave advances in single cell analyses, Guest Edited by Amy Herr (University of California, Berkeley, USA) Takehiko Kitamori (University of Tokyo, Japan), Ulf Landegren (Uppsala University, Sweden) and Masood Kamali-Moghaddam (Uppsala University, Sweden).

Single-cell multi-omics analysis helps characterize multiple layers of molecular features at a single-cell scale to provide insights into cellular processes and functions.

We provide a vision for integrating pioneering mass spectrometry technologies for future applications in single cell analysis.

Single cell metabolomics using mass spectrometry can contribute to understanding biological activities in health and disease.

Non-destructive spatial characterization of lipid droplets using coherent Raman scattering microscopy and computational image analysis algorithms at the single-cell level.

Scanning electrochemical microscopy (SECM) offers single live cell activities along its topography toward cellular physiology and pathology.

This review highlights the advances in single cell manipulation and biochemical analysis on microfluidics over the past three years.

We review the state of the art in HTS using mass spectrometry with minimal sample preparation from complex biological matrices. We focus on industrial and biotechnological applications.

Compared with the conventional ensemble averaged measurements, single object analysis with optical microscopy can obtain the heterogeneous behavior of many individual objects, avoiding false judgment. Moreover, higher spatial and temporal resolution has been achieved by various optical imaging technologies.

Cells are the most basic structural units that play vital roles in the functioning of living organisms.

Recent advances in single-cell analysis have unraveled substantial heterogeneity among seemingly identical cells at genomic and transcriptomic levels.

Advances in microfluidic techniques have prompted researchers to study the inherent heterogeneity of single cells in cell populations.

Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.

This paper presents a crossing constriction channel-based microfluidic system for high-throughput characterization of specific membrane capacitance (C_sm) and cytoplasm conductivity (σ_cy) of single cells.

Detection and characterization of microorganisms is essential for both clinical diagnostics and environmental studies.

This study focuses on characterizing the aging process of red blood cells by correlating the loss of hemoglobin and the translocation of phosphatidylserine (PS) in expired human red blood cells, hRBCs.

In situ capillary microsampling with capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry (MS) enabled the characterization of cationic and anionic metabolites in single cells in complex tissues and organisms, such as the live frog (X. laevis) embryo.

With lethal opportunistic fungal infections on the rise, it is imperative to explore new methods to examine virulence mechanisms.

A simple, cost-effective liquid sample holder to perform single live cell analysis under an IR microscope.

Two-color DNA physical map for efficient identification of single DNA molecules.

The simple and rapid method for isolation of single-adherent cells from a culture dish was developed and applied to genetic analysis of single-cells.

Single cell secretion studies have provided valuable insight into population heterogeneity, but low detection sensitivity requires cells to secrete thousands of molecules. We employed semiconductor quantum dots and a new single particle imaging approach to improve detection sensitivity to only a few secreted molecules per cell.

Prepending surface receptor immunostaining with single-cell polyacrylamide gel electrophoresis provides a new tool with which to understand how localization of surface receptor proteins controls the complex regulatory systems in single cells.

We demonstrate the first spectrum of cyclopropane fatty acid and track its presence in yeast using Raman spectroscopy and PLS-DA.

Fixation of fluorescent sphingolipid-loaded cells enables cell metabolism and assay readout to be separated in time and space.

Single-cell Raman microspectroscopy to detect phenylalanine as a potential biomarker for mitochondrial dysfunction and chronic fatigue syndrome.

Electrosyringe-assisted electrospray mass spectrometry (MS) is established for the first time to achieve intracellular sampling from one axon or dendrite in living neurons for mass spectrometric analysis.

Phospho-SISPROT achieves highly sensitive phosphoproteome analysis from lower than 20 μg of cell lysates.