Issue 1, 2018

Sample preparation for polar metabolites in bioanalysis

Abstract

Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about the actual practice and issues of hydrophilic metabolites' extraction, including new solutions and perspectives to improve their phase transfer from a complex biological sample to a clean extract prior to analysis.

Graphical abstract: Sample preparation for polar metabolites in bioanalysis

Article information

Article type
Minireview
Submitted
10 aug 2017
Accepted
27 nov 2017
First published
27 nov 2017

Analyst, 2018,143, 16-20

Sample preparation for polar metabolites in bioanalysis

N. Drouin, S. Rudaz and J. Schappler, Analyst, 2018, 143, 16 DOI: 10.1039/C7AN01333G

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