Issue 24, 2018

ATR-FTIR spectroscopy shows changes in ovarian cancer cells after incubation with novel organoamidoplatinum(ii) complexes

Abstract

Attenuated Total Reflection Fourier Transform Infrared (ATR-FT-IR) spectroscopy has been applied to compare the effect of the new organoamidoplatinum(II) complexes [Pt{NH(p-HC6F4)CH2CH2N(p-HC6F4)}(py)(O2CR)] (R = C6F4 or 2,4,6-Me3C6H2) with cisplatin on cells from one cisplatin-sensitive ovarian cancer cell line (A2780) and one cisplatin-resistant ovarian cancer cell line (A2780R). After incubation of the cells with cisplatin, 1 and 2 for 48 hours, distinct changes were found in the ATR-FT-IR spectra. Comparison of the second derivative spectra suggests that 1 and 2 induce similar chemical changes in both cell lines, A2780 and A2780R, while cisplatin had a slight effect on A2780 and A2780R cells. Furthermore, drugs 1 and 2 result in changes to the phosphodiester and polysaccharide bands in the spectra. This work shows how ATR-FT-IR can be applied to monitor the effects of organoamidoplatinum(II) complexes on cisplatin-sensitive and cisplatin-resistant cell lines providing potential information on how drugs affect the cellular metabolism.

Graphical abstract: ATR-FTIR spectroscopy shows changes in ovarian cancer cells after incubation with novel organoamidoplatinum(ii) complexes

Supplementary files

Article information

Article type
Paper
Submitted
12 aug 2018
Accepted
02 nov 2018
First published
05 nov 2018

Analyst, 2018,143, 6087-6094

ATR-FTIR spectroscopy shows changes in ovarian cancer cells after incubation with novel organoamidoplatinum(II) complexes

K. Al-Jorani, A. Rüther, R. Haputhanthri, G. B. Deacon, H. L. Li, C. Cullinane and B. R. Wood, Analyst, 2018, 143, 6087 DOI: 10.1039/C8AN01558A

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