Issue 11, 2015

In vivo visualization of osteoarthritic hypertrophic lesions

Abstract

Osteoarthritis (OA) is one of the most common diseases in the aging population. While disease progress in humans is monitored indirectly by X-ray or MRI, small animal OA lesions detection always requires surgical intervention and histology. Here we introduce bimodal MR/NIR probes based on cartilage-targeting 1,4,7,10-tetraazacyclododecane 1,4,7,10-tetraacetic acid amide (DOTAM) that are directly administered to the joint cavity. We demonstrate applications in healthy and diseased rat joints by MRI in vivo. The same joints are inspected post-mortem by fluorescence microscopy, showing not only the precise location of the reagents but also revealing details such as focal cartilage damage and chondrophyte or osteophyte formation. This allows for determining the distinct pathological state of the disease and the regeneration capability of the animal model and will help to correctly assess the effect of potential disease modifying OA drugs (DMOADs) in the future.

Graphical abstract: In vivo visualization of osteoarthritic hypertrophic lesions

Supplementary files

Article information

Article type
Edge Article
Submitted
10 apr 2015
Accepted
12 aug 2015
First published
12 aug 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2015,6, 6256-6261

Author version available

In vivo visualization of osteoarthritic hypertrophic lesions

H. Hu, N. Lim, H. Juretschke, D. Ding-Pfennigdorff, P. Florian, M. Kohlmann, A. Kandira, J. Peter von Kries, J. Saas, K. A. Rudolphi, K. U. Wendt, H. Nagase, O. Plettenburg, M. Nazare and C. Schultz, Chem. Sci., 2015, 6, 6256 DOI: 10.1039/C5SC01301A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements