Issue 19, 2019

Device for whole genome sequencing single circulating tumor cells from whole blood

Abstract

Whole-genome sequencing on circulating tumor cells (CTCs) at the single cell level has recently been found helpful for precision medicine, as the oncogenic profiles of single CTCs are useful for discovering oncogenic mutation heterogeneities and guiding/adjusting cancer treatment. To overcome the limits of existing methods of single CTC sequencing, in which CTC enrichment, identification and gene amplification are performed by discrete modules, this study presents a novel method in which all processing steps from blood sample collection to preparation of gene amplification products for sequencers are finished in a single microfluidic chip. This microfluidic chip comprehensively performs blood filtering, CTC enrichment, CTC identification/isolation, CTC lysis and whole genome amplification (WGA) at the single cell level. By sequencing single CTCs from clinical blood samples with pointing key driver and drug-resistance mutations, the novel microfluidic chip was validated to be capable of genetically profiling single CTCs with minimum cell loss/human labor, and more importantly, high accuracy and repeatability, which are crucial factors for promoting clinical application of single CTC sequencing.

Graphical abstract: Device for whole genome sequencing single circulating tumor cells from whole blood

Supplementary files

Article information

Article type
Paper
Submitted
19 mai 2019
Accepted
19 aug 2019
First published
19 aug 2019

Lab Chip, 2019,19, 3168-3178

Device for whole genome sequencing single circulating tumor cells from whole blood

R. Li, F. Jia, W. Zhang, F. Shi, Z. Fang, H. Zhao, Z. Hu and Z. Wei, Lab Chip, 2019, 19, 3168 DOI: 10.1039/C9LC00473D

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