Hot off the press

Robert A. Hill and Andrew Sutherland

Nat. Prod. Rep., 2021, 38, 2139
DOI: 10.1039/D1NP90046C

Expanding the fluorescence toolkit: molecular design, synthesis and biological application of unnatural amino acids

Olivia Marshall and Andrew Sutherland

Chem. Sci., 2025, 16, 16414
DOI: 10.1039/D5SC05745K

Genetically encoded fluorescent unnatural amino acids and FRET probes for detecting deubiquitinase activities

Manjia Li, Feifei Wang, Long Yan, Minghao Lu, Yuqing Zhang and Tao Peng

Chem. Commun., 2022, 58, 10186
DOI: 10.1039/D2CC03623A

Selective fluorescent labeling of cellular proteins and its biological applications

Joo Hee Choi, Sooin Kim, On-Yu Kang, Seong Yun Choi, Ji Young Hyun, Hyun Soo Lee and Injae Shin

Chem. Soc. Rev., 2024, 53, 9446
DOI: 10.1039/D4CS00094C

FRETing about the details: Case studies in the use of a genetically encoded fluorescent amino acid for distance‐dependent energy transfer

Michael B. Cory, Chloe M. Jones, Kyle D. Shaffer, Yarra Venkatesh, Sam Giannakoulias, Ryann M. Perez, Marshall G. Lougee, Eshe Hummingbird, Vinayak V. Pagar, Christina M. Hurley, Allen Li, Robert H. Mach, Rahul M. Kohli and E. James Petersson

Protein Science, 2023, 32
DOI: 10.1002/pro.4633

Noncanonical Amino Acid Tools and Their Application to Membrane Protein Studies

Chiara De Faveri, Jordan M. Mattheisen, Thomas P. Sakmar and Irene Coin

Chem. Rev., 2024, 124, 12498
DOI: 10.1021/acs.chemrev.4c00181

Biomolecular simulation based machine learning models accurately predict sites of tolerability to the unnatural amino acid acridonylalanine

Sam Giannakoulias, Sumant R. Shringari, John J. Ferrie and E. James Petersson

Sci Rep, 2021, 11
DOI: 10.1038/s41598-021-97965-2

Rendering Proteins Fluorescent Inconspicuously: Genetically Encoded 4‐Cyanotryptophan Conserves Their Structure and Enables the Detection of Ligand Binding Sites

Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Edan Habel, Nathan Paul, Rebecca L. Frkic, Colin J. Jackson, Thomas Huber and Gottfried Otting

Angew Chem Int Ed, 2025, 64
DOI: 10.1002/anie.202421000

Voltage sensor conformations induced by LQTS-associated mutations in hERG potassium channels

Aaron N. Chan, Co D. Quach, Lucas J. Handlin, Erin N. Lessie, Emad Tajkhorshid and Gucan Dai

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-62472-9

Cracking the Code: Reprogramming the Genetic Script in Prokaryotes and Eukaryotes to Harness the Power of Noncanonical Amino Acids

Cosimo Jann, Sabrina Giofré, Rajanya Bhattacharjee and Edward A. Lemke

Chem. Rev., 2024, 124, 10281
DOI: 10.1021/acs.chemrev.3c00878

Recent Advances in Fluorescence Imaging by Genetically Encoded Non-canonical Amino Acids

Sanghee Lee, Jonghoon Kim and Minseob Koh

Journal of Molecular Biology, 2022, 434, 167248
DOI: 10.1016/j.jmb.2021.167248

Noncanonical Amino Acid Incorporation in Animals and Animal Cells

Joo-Chan Kim, YouJin Kim, Suho Cho and Hee-Sung Park

Chem. Rev., 2024, 124, 12463
DOI: 10.1021/acs.chemrev.3c00955

Dual Noncanonical Amino Acid Incorporation Enabling Chemoselective Protein Modification at Two Distinct Sites in Yeast

Priyanka Lahiri, Meghan S. Martin, Briana R. Lino, Rebecca A. Scheck and James A. Van Deventer

Biochemistry, 2023, 62, 2098
DOI: 10.1021/acs.biochem.2c00711

Combining non-canonical amino acid mutagenesis and native chemical ligation for multiply modifying proteins: A case study of α-synuclein post-translational modifications

Ana Galesic, Buyan Pan, Jennifer Ramirez, Elizabeth Rhoades, Matthew R. Pratt and E. James Petersson

Methods, 2023, 218, 101
DOI: 10.1016/j.ymeth.2023.08.002

Cellular and Biophysical Applications of Genetic Code Expansion

Han Bin Yi, Seungeun Lee, Kyungdeok Seo, Hyeongjo Kim, Minah Kim and Hyun Soo Lee

Chem. Rev., 2024, 124, 7465
DOI: 10.1021/acs.chemrev.4c00112

Design and Evolution of a Phosphorescent Protein via the Proximal Encoding of Lanthanide and the Antenna Chromophore

Jinyu Wang, Xingfeng Liu, Kaitong Li, Tao Shi, Qianqian Xu, Tiantian Peng, Qingjun Huang, Ziqi Gao, Hongqi Zhou, Wei Lu and Jie Wang

J. Am. Chem. Soc., 2025, 147, 15205
DOI: 10.1021/jacs.5c00199

Biosynthesis, Engineering, and Delivery of Selenoproteins

David E. Wright and Patrick O’Donoghue

IJMS, 2023, 25, 223
DOI: 10.3390/ijms25010223

Visualizing the complexity of proteins in living cells with genetic code expansion

Kanokpol Aphicho, Narongyot Kittipanukul and Chayasith Uttamapinant

Current Opinion in Chemical Biology, 2022, 66, 102108
DOI: 10.1016/j.cbpa.2021.102108

Directed evolution of aminoacyl-tRNA synthetases through in vivo hypermutation

Yuichi Furuhata, Gordon Rix, James A. Van Deventer and Chang C. Liu

Nat Commun, 2025, 16
DOI: 10.1038/s41467-025-60120-w

Reaching New Heights in Genetic Code Manipulation with High Throughput Screening

Briana R. Lino, Sean J. Williams, Michelle E. Castor and James A. Van Deventer

Chem. Rev., 2024, 124, 12145
DOI: 10.1021/acs.chemrev.4c00329

FRET Materials for Biosensing and Bioimaging

Ruifang Su, Laura Francés-Soriano, P. Iyanu Diriwari, Muhammad Munir, Lucie Haye, Thomas J. Sørensen, Sebastián A. Díaz, Igor L. Medintz and Niko Hildebrandt

Chem. Rev., 2025
DOI: 10.1021/acs.chemrev.5c00386

Genetic Code Expansion for Mechanistic Studies in Ion Channels: An (Un)natural Union of Chemistry and Biology

Daniel T. Infield, Miranda E. Schene, Jason D. Galpin and Christopher A. Ahern

Chem. Rev., 2024, 124, 11523
DOI: 10.1021/acs.chemrev.4c00306

Characterising ion channel structure and dynamics using fluorescence spectroscopy techniques

Shreyas Bhat and Rikard Blunck

Biochemical Society Transactions, 2022, 50, 1427
DOI: 10.1042/BST20220605

Improved Large-Scale Synthesis of Acridonylalanine for Diverse Peptide and Protein Applications

Jason G. Marmorstein, Vinayak V. Pagar, Eshe Hummingbird, Ibrahim G. Saleh, Hoang Anh T. Phan, Yanan Chang, Kyle D. Shaffer, Yarra Venkatesh, Ivan J. Dmochowski, Kathleen J. Stebe and E. James Petersson

Bioconjugate Chem., 2024, 35, 1913
DOI: 10.1021/acs.bioconjchem.4c00411

Chemical tags and beyond: Live‐cell protein labeling technologies for modern optical imaging

Dilizhatai Saimi and Zhixing Chen

Smart Molecules, 2023, 1
DOI: 10.1002/smo.20230002

Development of artificial photosystems based on designed proteins for mechanistic insights into photosynthesis

Gonzalo Pérez Serrano, Claudia F. Echavarría and Sara H. Mejias

Protein Science, 2024, 33
DOI: 10.1002/pro.5164

Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming

Daniel L. Dunkelmann and Jason W. Chin

Chem. Rev., 2024, 124, 11008
DOI: 10.1021/acs.chemrev.4c00243

Update of the Pyrrolysyl-tRNA Synthetase/tRNA Pyl Pair and Derivatives for Genetic Code Expansion

Xuemei Gong, Haolin Zhang, Yue Shen, Xian Fu and Julie A. Maupin-Furlow

J Bacteriol, 2023, 205
DOI: 10.1128/jb.00385-22

Strategies to Expand the Genetic Code of Mammalian Cells

Arianna O. Osgood, Zeyi Huang, Kaitlyn H. Szalay and Abhishek Chatterjee

Chem. Rev., 2025, 125, 2474
DOI: 10.1021/acs.chemrev.4c00730

Genetic Code Expansion: Recent Developments and Emerging Applications

Yujia Huang, Pan Zhang, Haoyu Wang, Yan Chen, Tao Liu and Xiaozhou Luo

Chem. Rev., 2025, 125, 523
DOI: 10.1021/acs.chemrev.4c00216

Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetases for Structurally Diverse Non-Canonical Amino Acids

Savanna Avila-Crump, Marcus L. Hemshorn, Chloe M. Jones, Lea Mbengi, Kyle Meyer, Joshua A. Griffis, Subhashis Jana, Grace E. Petrina, Vinayak V. Pagar, P. Andrew Karplus, E. James Petersson, John J. Perona, Ryan A. Mehl and Richard B. Cooley

ACS Chem. Biol., 2022, 17, 3458
DOI: 10.1021/acschembio.2c00639

An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

William N Zagotta, Brandon S Sim, Anthony K Nhim, Marium M Raza, Eric GB Evans, Yarra Venkatesh, Chloe M Jones, Ryan A Mehl, E James Petersson and Sharona E Gordon

eLife, 2021, 10
DOI: 10.7554/eLife.70236

Hybrid Small-Molecule/Protein Fluorescent Probes

Masafumi Minoshima, Shahi Imam Reja, Ryu Hashimoto, Kohei Iijima and Kazuya Kikuchi

Chem. Rev., 2024, 124, 6198
DOI: 10.1021/acs.chemrev.3c00549

Expanding the Genetic Code of Bioelectrocatalysis and Biomaterials

Yonatan Chemla, Federico Kaufman, Miriam Amiram and Lital Alfonta

Chem. Rev., 2024, 124, 11187
DOI: 10.1021/acs.chemrev.4c00077

Rendering Proteins Fluorescent Inconspicuously: Genetically Encoded 4‐Cyanotryptophan Conserves Their Structure and Enables the Detection of Ligand Binding Sites

Haocheng Qianzhu, Elwy H. Abdelkader, Adarshi P. Welegedara, Edan Habel, Nathan Paul, Rebecca L. Frkic, Colin J. Jackson, Thomas Huber and Gottfried Otting

Angewandte Chemie, 2025, 137
DOI: 10.1002/ange.202421000

Genetic code expansion, click chemistry, and light-activated PI3K reveal details of membrane protein trafficking downstream of receptor tyrosine kinases

Duk-Su Koh, Anastasiia Stratiievska, Subhashis Jana, Shauna C Otto, Teresa M Swanson, Anthony Nhim, Sara Carlson, Marium Raza, Ligia Araujo Naves, Eric N Senning, Ryan A Mehl and Sharona E Gordon

eLife, 2024, 12
DOI: 10.7554/eLife.91012.3