Issue 11, 2023

Electro-mechanical coupling directs endothelial activities through intracellular calcium ion deployment

Abstract

Conversion between mechanical and electrical cues is usually considered unidirectional in cells with cardiomyocytes being an exception. Here, we discover a material-induced external electric field (Eex) triggers an electro-mechanical coupling feedback loop in cells other than cardiomyocytes, human umbilical vein endothelial cells (HUVECs), by opening their mechanosensitive Piezo1 channels. When HUVECs are cultured on patterned piezoelectric materials, the materials generate Eex (confined at the cellular scale) to polarize intracellular calcium ions ([Ca2+]i), forming a built-in electric field (Ein) opposing Eex. Furthermore, the [Ca2+]i polarization stimulates HUVECs to shrink their cytoskeletons, activating Piezo1 channels to induce influx of extracellular Ca2+ that gradually increases Ein to balance Eex. Such an electro-mechanical coupling feedback loop directs pre-angiogenic activities such as alignment, elongation, and migration of HUVECs. Activated calcium dynamics during the coupling further modulate the downstream angiogenesis-inducing eNOS/NO pathway. These findings lay a foundation for developing new ways of electrical stimulation-based disease treatment.

Graphical abstract: Electro-mechanical coupling directs endothelial activities through intracellular calcium ion deployment

Supplementary files

Article information

Article type
Communication
Submitted
06 jul. 2023
Accepted
06 sep. 2023
First published
26 sep. 2023

Mater. Horiz., 2023,10, 4903-4913

Electro-mechanical coupling directs endothelial activities through intracellular calcium ion deployment

C. Li, P. Yu, Z. Wang, C. Long, C. Xiao, J. Xing, B. Dong, J. Zhai, L. Zhou, Z. Zhou, Y. Wang, W. Zhu, G. Tan, C. Ning, Y. Zhou and C. Mao, Mater. Horiz., 2023, 10, 4903 DOI: 10.1039/D3MH01049J

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