Issue 31, 2022

Development of an ATP-independent bioluminescent probe for detection of extracellular hydrogen peroxide

Abstract

This work reports a new ATP-independent bioluminescent probe (bor-DTZ) for detecting hydrogen peroxide that is compatible with the Nanoluciferase enzyme. The probe is designed with an arylboronate ester protecting group appended to a diphenylterazine core via a self-immolative phenolate linker. Reaction with hydrogen peroxide reveals diphenylterazine, which can then react with Nanoluciferase to produce a detectable bioluminescent signal. Bor-DTZ shows a dose-dependent response to hydrogen peroxide and selectivity over other biologically relevant reactive oxygen species and can be applied to detect either intra- or extracellular species. We further demonstrate the ability of this platform to monitor fluxes in extracellular hydrogen peroxide in a breast cancer cell line in response to the anticancer treatment, cisplatin.

Graphical abstract: Development of an ATP-independent bioluminescent probe for detection of extracellular hydrogen peroxide

  • This article is part of the themed collection: New Talent

Supplementary files

Article information

Article type
Paper
Submitted
03 mar. 2022
Accepted
05 may. 2022
First published
12 may. 2022

Org. Biomol. Chem., 2022,20, 6231-6238

Author version available

Development of an ATP-independent bioluminescent probe for detection of extracellular hydrogen peroxide

J. J. O'Sullivan and M. C. Heffern, Org. Biomol. Chem., 2022, 20, 6231 DOI: 10.1039/D2OB00436D

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