Issue 18, 2021

A feasible self-assembled near-infrared fluorescence sensor for acid phosphatase detection and cell imaging

Abstract

The single signal amplification strategy is significant for detecting various disease biomarkers but is restricted by its limited accuracy. The multi-signal and multi-mode methods have overcome this deficiency. Acid phosphatase (ACP) is an important intracellular enzyme but one-step cell imaging material-based probes are scarce for ACP. Herein, we designed a one-step self-assembled polymer probe using neutral red (NR), modified-(pyridoxal-5′-phosphate (PLP)) and Eu3+. The polymer exhibited non-emission and excellent stability. Upon the catalytic hydrolysis reaction of ACP, the polymer exhibited two strong fluorescence signals at 373 nm and 613 nm and an appreciable decline of absorbance at 395 nm. The probe has excellent selectivity and higher sensitivity with a limit of detection as low as 0.02 mU mL−1. It possesses favorable biocompatibility and has been successfully used to detect and image intracellular ACP in several living cells.

Graphical abstract: A feasible self-assembled near-infrared fluorescence sensor for acid phosphatase detection and cell imaging

Supplementary files

Article information

Article type
Paper
Submitted
07 jul. 2021
Accepted
27 jul. 2021
First published
03 ago. 2021

Analyst, 2021,146, 5558-5566

A feasible self-assembled near-infrared fluorescence sensor for acid phosphatase detection and cell imaging

S. Li, Y. Wang, S. Mu, J. Zhang, X. Liu, S. F. A. Rizvi, H. Zhang, N. Ding and L. Wu, Analyst, 2021, 146, 5558 DOI: 10.1039/D1AN01218E

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