Continuous flow biocatalysis: production and in-line purification of amines by immobilised transaminase from Halomonas elongata

Abstract

The continuous flow synthesis of a series of amines was successfully achieved by exploiting the enhanced stability and broad substrate scope of an immobilised transaminase from Halomonas elongata (HEWT). A series of substrates were tested in flow reactors and transformed into the corresponding amines in good to excellent yields. The process was implemented with an integrated in-line purification step for the recovery of the pure amines.