Issue 14, 2016

Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells

Abstract

By combining super-resolution photoactivation localization microscopy with single particle tracking, we have visualized the endocytic process in the live-cell environment with nanoparticles (NPs) of different size and surface functionalization. This allowed us to analyze the dynamics of NPs interacting with cells with high spatial and temporal resolution. We identified two distinctly different types of pathways by which NPs are internalized via clathrin-coated pits (CCPs). Predominantly, NPs first bind to the membrane and, subsequently, CCPs form at this site. However, there are also instances where a NP diffuses on the membrane and utilizes a preformed CCP. Moreover, we have applied this new method to further explore the effects of size and surface functionalization on the NP dynamics on the plasma membrane and the ensuing endocytosis.

Graphical abstract: Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells

Supplementary files

Article information

Article type
Communication
Submitted
22 feb. 2016
Accepted
14 mar. 2016
First published
16 mar. 2016

Nanoscale, 2016,8, 7423-7429

Super-resolution imaging-based single particle tracking reveals dynamics of nanoparticle internalization by live cells

Y. Li, L. Shang and G. U. Nienhaus, Nanoscale, 2016, 8, 7423 DOI: 10.1039/C6NR01495J

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