Issue 22, 2023

SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

Abstract

Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.

Graphical abstract: SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

Supplementary files

Article information

Article type
Communication
Submitted
02 dic. 2022
Accepted
13 feb. 2023
First published
14 feb. 2023
This article is Open Access
Creative Commons BY license

Chem. Commun., 2023,59, 3249-3252

SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids

W. H. Skinner, N. Robinson, G. R. Hardisty, H. Fleming, A. Geddis, M. Bradley, R. D. Gray and C. J. Campbell, Chem. Commun., 2023, 59, 3249 DOI: 10.1039/D2CC06582G

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