Issue 11, 2022

A light-controlled biocatalytic system for precise regulation of enzymatic decarboxylation

Abstract

Precise control of a biocatalytic system is highly important but remains a major challenge. Herein, we developed a light-controlled enzyme production-biocatalysis one-pot system by combination of photocatalysis and photogenetic-regulated enzyme production. As proof-of-principle, a photoenzyme in an Escherichia coli-based biocatalyst was produced and restored by gene expression in the dark for decarboxylation under illumination. The kinetic resolution of racemic phosphinothricin with an intermittent illumination model displayed higher conversion (49%) and excellent stereoselectivity (ee = 98%), compared to a continuous illumination model (conv. = 44%; ee = 71%).

Graphical abstract: A light-controlled biocatalytic system for precise regulation of enzymatic decarboxylation

Supplementary files

Article information

Article type
Communication
Submitted
13 mar. 2022
Accepted
01 may. 2022
First published
02 may. 2022

Catal. Sci. Technol., 2022,12, 3421-3425

A light-controlled biocatalytic system for precise regulation of enzymatic decarboxylation

F. Cheng, D. Wu, X. Liang, C. Wang, J. Weng, S. Zou, J. Xu, Y. Xue and Y. Zheng, Catal. Sci. Technol., 2022, 12, 3421 DOI: 10.1039/D2CY00480A

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