Issue 5, 2022

The laccase mediator system at carbon nanotubes for anthracene oxidation and femtomolar electrochemical biosensing

Abstract

We investigated the use of POXA1b laccase from Pleurotus ostreatus for the oxidation of anthracene into anthraquinone. We show that different pathways can occur depending on the nature of the redox mediator combined to laccase, leading to different structural isomers. The laccase combined with 2,2′-azine-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) leads to the formation of 1,4-anthraquinone and/or 1,2-anthraquinone. The unprecedented role of carbon nanotubes (CNTs) as redox mediators for oxidation of anthracene into 9,10-anthraquinone is shown and corroborated by density-functional theory (DFT) calculations. Owing to the efficient adsorption of anthraquinones at CNT electrodes, anthracene can be detected with low limit-of-detection using either laccase in solution, CNT-supported laccase or laccase immobilized at magnetic beads exploiting the adhesive property of a chimeric hydrophobin-laccase.

Graphical abstract: The laccase mediator system at carbon nanotubes for anthracene oxidation and femtomolar electrochemical biosensing

Supplementary files

Article information

Article type
Paper
Submitted
18 nov. 2021
Accepted
02 feb. 2022
First published
03 feb. 2022
This article is Open Access
Creative Commons BY license

Analyst, 2022,147, 897-904

The laccase mediator system at carbon nanotubes for anthracene oxidation and femtomolar electrochemical biosensing

I. Sorrentino, M. Carrière, H. Jamet, I. Stanzione, A. Piscitelli, P. Giardina and A. Le Goff, Analyst, 2022, 147, 897 DOI: 10.1039/D1AN02091A

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