Issue 34, 2021

Urate oxidase loaded in PCN-222(Fe) with peroxidase-like activity for colorimetric detection of uric acid

Abstract

In the past two decades, the number of reports on the construction of uric acid (UA) sensors has increased dramatically, as it is a vital factor in the diagnosis of physiological functions and diseases. Among these sensors, cascade colorimetric detection based on peroxidase mimics has received great attention owing to the advantages of easier operation and more intuitive results. Herein, we report a simple UA detection method via the integration of urate oxidase (UOx) and PCN-222(Fe) with peroxidase-like activity, in which UOx is immobilized in PCN-222(Fe) by physical adsorption. UOx could catalyze the UA oxidation to produce H2O2, and then PCN-222(Fe) catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to generate a blue product with a maximum absorbance at 652 nm. Under optimal conditions, the increasing absorbance at 652 nm was proportional to the UA concentration. The linear range for UA detection was 10–800 μM with a limit of detection of 3.5 μM, and the cascade system has been successfully utilised for the detection of UA in human serum samples to demonstrate the accuracy of the proposed method. In conclusion, the cascade catalytic system based on the immobilization of UOx in PCN-222(Fe) has the potential to be used as a rapid and sensitive sensor for UA detection.

Graphical abstract: Urate oxidase loaded in PCN-222(Fe) with peroxidase-like activity for colorimetric detection of uric acid

Supplementary files

Article information

Article type
Paper
Submitted
25 jun. 2021
Accepted
28 jul. 2021
First published
28 jul. 2021

J. Mater. Chem. B, 2021,9, 6811-6817

Urate oxidase loaded in PCN-222(Fe) with peroxidase-like activity for colorimetric detection of uric acid

X. Liang, Y. Chen, K. Wen, H. Han and Q. Li, J. Mater. Chem. B, 2021, 9, 6811 DOI: 10.1039/D1TB01424B

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