Issue 17, 2021

A novel p-dimethylaminophenylether-based fluorescent probe for the detection of native ONOO in cells and zebrafish

Abstract

Peroxynitrite (ONOO) is a highly reactive substance, and plays an essential part in maintaining cellular homeostasis. It is crucial to monitor the ONOO level in cells in normal and abnormal states. We introduced a p-dimethylaminophenylether-based fluorescent probe PDPE-PN, which could be synthesized readily. The new probe had prominent sensitivity and specificity, and a fast response towards ONOO. The spectral performance of probe PDPE-PN was outstanding and the limit of detection was 69 nM. Probe PDPE-PN with low toxicity was applied to detect endogenous/exogenous ONOO in RAW 264.7 macrophages and zebrafish. Importantly, successful application of the new receptor opens up new ideas for the design of ONOO probes.

Graphical abstract: A novel p-dimethylaminophenylether-based fluorescent probe for the detection of native ONOO− in cells and zebrafish

Supplementary files

Article information

Article type
Paper
Submitted
10 abr. 2021
Accepted
15 jul. 2021
First published
15 jul. 2021

Analyst, 2021,146, 5264-5270

A novel p-dimethylaminophenylether-based fluorescent probe for the detection of native ONOO in cells and zebrafish

W. Sheng, K. Wang, N. Gao, L. Wang, R. Wang, X. Zhang, X. Chen, Y. Zhang, B. Zhu and K. Liu, Analyst, 2021, 146, 5264 DOI: 10.1039/D1AN00608H

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