Issue 12, 2020

Selective glucose sensing in complex media using a biomimetic receptor

Abstract

Glucose is a key biomedical analyte, especially relevant to the management of diabetes. Current methods for glucose determination rely on the enzyme glucose oxidase, requiring specialist instrumentation and suffering from redox-active interferents. In a new approach, a powerful and highly selective achiral glucose receptor is mixed with a sample, L-glucose is added, and the induced CD spectrum is measured. The CD signal results from competition between the enantiomers, and is used to determine the D-glucose content. The involvement of L-glucose doubles the signal range from the CD spectrometer and allows sensitivity to be adjusted over a wide dynamic range. It also negates medium effects, which must be equal for both enantiomers. The method has been demonstrated with human serum, pre-filtered to remove proteins, giving results which closely match the standard biochemical procedures, as well as a cell culture medium and a beer sample containing high (70 mM) and low (0.4 mM) glucose concentrations respectively.

Graphical abstract: Selective glucose sensing in complex media using a biomimetic receptor

Supplementary files

Article information

Article type
Edge Article
Submitted
25 oct. 2019
Accepted
23 feb. 2020
First published
25 feb. 2020
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2020,11, 3223-3227

Selective glucose sensing in complex media using a biomimetic receptor

R. A. Tromans, S. K. Samanta, A. M. Chapman and A. P. Davis, Chem. Sci., 2020, 11, 3223 DOI: 10.1039/C9SC05406E

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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