Issue 3, 2019

Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting

Abstract

Three-dimensional (3D) bioprinting is important in the development of complex tissue structures for tissue engineering and regenerative medicine. However, the materials used for bioprinting, referred to as bioinks, must have a balance between a high viscosity for rapid solidification after extrusion and low shear force for cytocompatibility, which is difficult to achieve. Here, a novel bioink consisting of poly(ethylene glycol) (PEG) microgels prepared via off-stoichiometry thiol–ene click chemistry is introduced. Importantly, the microgel bioink is easily extruded, exhibits excellent stability after printing due to interparticle adhesion forces, and can be photochemically annealed with a second thiol–ene click reaction to confer long-term stability to printed constructs. The modularity of the bioink is also an advantage, as the PEG microgels have highly tunable physicochemical properties. The low force required for extrusion and cytocompatibility of the thiol–ene annealing reaction also permit cell incorporation during printing with high viability, and cells are able to spread and proliferate in the interstitial spaces between the microgels after the constructs have been annealed. Overall, these results indicate that our microgel bioink is a promising and versatile platform that could be leveraged for bioprinting and regenerative manufacturing.

Graphical abstract: Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting

Supplementary files

Article information

Article type
Paper
Submitted
12 oct. 2018
Accepted
09 ene. 2019
First published
09 ene. 2019

Biomater. Sci., 2019,7, 1179-1187

Author version available

Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting

S. Xin, D. Chimene, J. E. Garza, A. K. Gaharwar and D. L. Alge, Biomater. Sci., 2019, 7, 1179 DOI: 10.1039/C8BM01286E

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