5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

Martina Janoušková; Zuzana Vaníková; Fabrizia Nici; Soňa Boháčová; Dragana Vítovská; Hana Šanderová; Michal Hocek; Libor Krásný

doi:10.1039/C7CC08053K

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5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase†

Martina Janoušková,‡^a Zuzana Vaníková,‡^b Fabrizia Nici,^b Soňa Boháčová,^b Dragana Vítovská,^a Hana Šanderová,^a Michal Hocek

*^bc and Libor Krásný

*^a

Author affiliations

* Corresponding authors

^a Dept. of Molecular Genetics of Bacteria, Institute of Microbiology of the Czech Academy of Sciences, CZ-14220 Prague 4, Czech Republic
E-mail: krasny@biomed.cas.cz

^b Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo nam. 2, 16610 Prague 6, Czech Republic
E-mail: hocek@uochb.cas.cz

^c Dept. of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, CZ-12843 Prague 2, Czech Republic

Abstract

DNA templates containing 5-hydroxymethyluracil or 5-hydroxymethylcytosine were used in an in vitro transcription assay with RNA polymerase from Escherichia coli. A strong enhancement of transcription was observed from DNA containing the Pveg promoter whereas a decrease was observed from DNA containing the rrnB P1 promoter, suggesting that they may act as epigenetic marks.

This article is part of the themed collection: Nucleic Acid Modifications

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DOI: https://doi.org/10.1039/C7CC08053K
Article type: Communication
Submitted: 18 oct. 2017
Accepted: 21 nov. 2017
First published: 22 nov. 2017
This article is Open Access

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Chem. Commun., 2017,53, 13253-13255

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5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

M. Janoušková, Z. Vaníková, F. Nici, S. Boháčová, D. Vítovská, H. Šanderová, M. Hocek and L. Krásný, Chem. Commun., 2017, 53, 13253 DOI: 10.1039/C7CC08053K

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Chemical Communications

5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase†

Abstract

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5-(Hydroxymethyl)uracil and -cytosine as potential epigenetic marks enhancing or inhibiting transcription with bacterial RNA polymerase

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