Issue 20, 2016

The effect of hypoxia and laminin-rich substrates on the proliferative behavior of human neural stem cells

Abstract

Human neural stem cells (hNSCs) possess an enormous potential to be utilized in novel cell-replacement therapies for neurodegenerative diseases and injuries. The hNSCs are a renewable source of cells with the capacity to generate the major cell types of the central nervous system (CNS). However, the translational potential of cell-based therapy is constrained due to the limited availability of scalable methods to rapidly expand numbers of stem cells in vitro. Here, we investigated the possible synergistic effect of oxygen concentration and substrate composition on hNSC growth. The hNSCs were cultured on six different substrates (i.e., collagen I, collagen IV, poly-L-ornithine, fibronectin, laminin, and Matrigel) under normoxic (21% oxygen concentration) and hypoxic (3% oxygen concentration) conditions and then total cell numbers were determined after 2 and 4 d. The percentages of cells undergoing proliferation (EdU+) and apoptosis (TUNEL+) varied with culture conditions, with a synergistic interaction between Matrigel substrate and hypoxia that resulted in the greatest number of hNSCs after 4 d compared to other conditions. These findings inform new methods to scale up NSC production by identifying potential substrate biomaterial design criteria as well as culture conditions that favor the generation of larger numbers of undifferentiated cells.

Graphical abstract: The effect of hypoxia and laminin-rich substrates on the proliferative behavior of human neural stem cells

  • This article is part of the themed collection: Stem Cells

Article information

Article type
Communication
Submitted
21 dic. 2015
Accepted
24 mar. 2016
First published
25 mar. 2016

J. Mater. Chem. B, 2016,4, 3509-3514

Author version available

The effect of hypoxia and laminin-rich substrates on the proliferative behavior of human neural stem cells

S. Sharee Ghourichaee and J. B. Leach, J. Mater. Chem. B, 2016, 4, 3509 DOI: 10.1039/C5TB02701B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements