Issue 24, 2016

Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system

Abstract

The combination of a microfluidic paper-based analytical device (μPAD) and digital image analysis is widely used for quantitative analysis with μPADs because of its easy and simple operation. Herein, we have demonstrated a quantitative analysis based on multiple color changes on a μPAD. The CIE L*a*b* color system was employed to analyse the digital images obtained with the μPAD. We made pH measurements using a universal pH-indicator showing multiple color changes for various pH values of aqueous test solutions. The detectable pH range of this method was wider than the typical grayscale-based image analysis, and we succeeded in the measurements for a wide pH range of 2–9.

Graphical abstract: Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system

Supplementary files

Article information

Article type
Communication
Submitted
21 jun. 2016
Accepted
27 oct. 2016
First published
28 oct. 2016
This article is Open Access
Creative Commons BY license

Analyst, 2016,141, 6507-6509

Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system

T. Komatsu, S. Mohammadi, L. S. A. Busa, M. Maeki, A. Ishida, H. Tani and M. Tokeshi, Analyst, 2016, 141, 6507 DOI: 10.1039/C6AN01409G

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