Issue 3, 2015

A thermostable transketolase evolved for aliphatic aldehyde acceptors

Abstract

Directed evolution of the thermostable transketolase from Geobacillus stearothermophilus based on a pH-based colorimetric screening of smart libraries yielded several mutants with up to 16-fold higher activity for aliphatic aldehydes and high enantioselectivity (>95% ee) in the asymmetric carboligation step.

Graphical abstract: A thermostable transketolase evolved for aliphatic aldehyde acceptors

Supplementary files

Article information

Article type
Communication
Submitted
25 oct. 2014
Accepted
18 nov. 2014
First published
18 nov. 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 480-483

A thermostable transketolase evolved for aliphatic aldehyde acceptors

D. Yi, T. Saravanan, T. Devamani, F. Charmantray, L. Hecquet and W. Fessner, Chem. Commun., 2015, 51, 480 DOI: 10.1039/C4CC08436E

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